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Invitrogen™ ProQuest™ Two-Hybrid System with Gateway™ Technology
Description
Includes
The ProQuest Two-Hybrid System includes yeast expression vectors pDEST32, pDEST22, and pEXP-AD502 for generation of GAL4 DNA Binding Domain and GAL4 Activation Domain fusion proteins, and LR Clonase II Enzyme Mix, a glycerol stock of the MaV203 yeast strain, as well as positive and negative controls for the two-hybrid assay.
- Reconstitutes a functional transcription factor that activates chromosomally integrated reporter genes where transcriptional activation is monitored by the growth of cells on selective media
- Allow rapid and easy generation of bait and prey constructs, and facilitate downstream application
- Low copy-number DBD (DNA Binding Domain) and AD (Activation Domain) vectors (ARS/CEN) to control over-expression and increase reproducibility
- Three different reporter genes (HIS3, URA3, and lacZ) with independent promoter regions to rapidly weed out false positives
- URA3 reporter gene allows for both positive and negative selection, enabling advanced two-hybrid techniques such as reverse two-hybrid
- A set of strong-to-weak Gateway-based control interactions to evaluate results and assess the strength of the interaction being tested
Protein-Protein Interactions, Proteins, Expression, Isolation and Analysis, Yeast Two-Hybrid
Specifications
Specifications
| Product Type | Two-Hybrid System |
| Reporter Gene | Beta-Gal (lacZ), HIS3, URA3 |
| Content And Storage | The ProQuest™ Two-Hybrid System includes yeast expression vectors pDEST™32, pDEST™22, and pEXP-AD502 for generation of GAL4 DNA Binding Domain and GAL4 Activation Domain fusion proteins, and LR Clonase™ II Enzyme Mix, a glycerol stock of the MaV203 yeast strain, as well as positive and negative controls for the two-hybrid assay. Store kit components at -80°C. Guaranteed stable for six months when properly stored. |
| Assay | Two-Hybrid Assay |
| Expression System | Yeast |
| Quantity | 1 kit |
| Product Line | Gateway™, ProQuest™ |
For Research Use Only. Not for use in diagnostic procedures.
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