Invitrogen™ pRSET A, B, & C Bacterial Expression Vectors

Description

Includes

20μg of each pRSET A, B and C vector is provided lyophilized. TOP10F' E. coli stab, BL21(DE3)pLysS E. coli stab, and BL21 pRSET/lacZ control E. coli stab are also included.

Designed for high-level prokaryotic expression controlled by the strong bacteriophage T7 promoter

• Expression is induced by production of T7 RNA polymerase in BL21(DE3) E. coli
• These cells also produce T7 lysozyme to reduce basal expression of target genes
• pRSET vector offers bacteriophage T7 promoter for high-level expression
• T7 gene 10 sequence to provide protein stability
• N-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an Anti-HisG Antibody
• N-terminal Xpress epitope for detection with the Anti-Xpress Antibody
• Enterokinase cleavage site for removal of fusion tag
• Each of three vectors provided (A, B, and C) has N-terminal tag coding sequence in a different reading frame relative to multiple cloning site to simplify in-frame cloning of the gene

Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Specifications

• Constitutive or Inducible System: Inducible
• Inducing Agent: IPTG
• Promoter: T7
• Product Type: Bacterial Expression Vector
• Selection Agent (Eukaryotic): None
• Content And Storage: 20 μg of each pRSET A, B, & C vector is provided lyophilized. TOP10F´, BL21(DE3)pLysS, and BL21 pRSET/lacZ control E. coli stabs are also included. Store E. coli stabs at 2–8°C. All components are guaranteed stable for 6 months when properly stored.
• Antibiotic Resistance Bacterial: Ampicillin (AmpR)
• Cleavage: EK (Enterokinase) Recognition Site
• Protein Tag: His Tag (6x)
• Cloning Method: Restriction Enzyme/MCS
• Quantity: 20 μg
• Vector: pRSET

For Research Use Only. Not for use in diagnostic procedures.