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Invitrogen™ pRSET A, B, & C Bacterial Expression Vectors

Catalog No. V35120
Encompass_Preferred
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Quantity:
20 μg

Includes

20μg of each pRSET A, B and C vector is provided lyophilized. TOP10F' E. coli stab, BL21(DE3)pLysS E. coli stab, and BL21 pRSET/lacZ control E. coli stab are also included.

Designed for high-level prokaryotic expression controlled by the strong bacteriophage T7 promoter

  • Expression is induced by production of T7 RNA polymerase in BL21(DE3) E. coli
  • These cells also produce T7 lysozyme to reduce basal expression of target genes
  • pRSET vector offers bacteriophage T7 promoter for high-level expression
  • T7 gene 10 sequence to provide protein stability
  • N-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an Anti-HisG Antibody
  • N-terminal Xpress epitope for detection with the Anti-Xpress Antibody
  • Enterokinase cleavage site for removal of fusion tag
  • Each of three vectors provided (A, B, and C) has N-terminal tag coding sequence in a different reading frame relative to multiple cloning site to simplify in-frame cloning of the gene

Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

TRUSTED_SUSTAINABILITY

Specifications

Constitutive or Inducible System Inducible
Inducing Agent IPTG
Promoter T7
Product Type Bacterial Expression Vector
Selection Agent (Eukaryotic) None
Content And Storage 20 μg of each pRSET A, B, & C vector is provided lyophilized. TOP10F´, BL21(DE3)pLysS, and BL21 pRSET/lacZ control E. coli stabs are also included. Store E. coli stabs at 2–8°C. All components are guaranteed stable for 6 months when properly stored.
Antibiotic Resistance Bacterial Ampicillin (AmpR)
Cleavage EK (Enterokinase) Recognition Site
Protein Tag His Tag (6x)
Cloning Method Restriction Enzyme/MCS
Quantity 20 μg
Vector pRSET
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For Research Use Only. Not for use in diagnostic procedures.

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