Invitrogen™ pTrcHis TOPO™ TA Expression Kit

Description

Includes

The pTrcHis-2-TOPO TA Expression Kit contains two boxes. The TOPO TA box contains 200ng of linearized, topoisomerase I-activated pTrcHis-TOPO or pTrcHis2-TOPO vector, sterile water, dNTPs, 10X PCR buffer, a control template and primers, primers for sequencing or PCR screening, and an expression control plasmid. The TOP10 box contains 21 x 50μL aliquots of chemically competent TOP10 E. coli, S.O.C. medium, and a supercoiled control plasmid.

Allow fast, efficient cloning of PCR products into prokaryotic expression vector for high-level, regulated expression from trc promoter

• Vector includes minicistron element for enhanced translation efficiency of eukaryotic proteins in E. coli
• Vector provided linearized and activated with topoisomerase I for 5-minute TOPO Cloning with >85% cloning efficiency
• N-terminal Xpress epitope for detection with an Anti-Xpress Antibody
• N-terminal polyhistidine (6xHis) tag for purification using nickel-chelating resin and detection with Anti-HisG Antibody
• Enterokinase cleavage site for efficient removal of N-terminal fusion tags
• C-terminal polyhistidine (6xHis) tag for purification using nickel-chelating resin and detection with Anti-His(C-term) Antibody

Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Specifications

• Constitutive or Inducible System: Inducible
• Inducing Agent: IPTG
• Promoter: Trc, lacO
• Product Type: TA Expression Kit
• Selection Agent (Eukaryotic): None
• Content And Storage: The pTrcHis- and pTrcHis2-TOPO™ TA Expression Kits each contain two boxes. The TOPO™ TA box contains 200 ng of linearized, topoisomerase I-activated pTrcHis-TOPO™ or pTrcHis2-TOPO™ vector, sterile water, dNTPs, 10X PCR buffer, a control template and primers, primers for sequencing or PCR screening, and an expression control plasmid. The One Shot™ TOP10 box contains twenty-one, 50-μl aliquots of chemically competent TOP10 E. coli, S.O.C. medium, and a supercoiled control plasmid. Store the TOPO TA Cloning™ box at -20°C. Store the One Shot™ box at -80°C. All reagents are guaranteed stable for 6 months when properly stored.
• Antibiotic Resistance Bacterial: Ampicillin (AmpR)
• Bacterial or Yeast Strain: TOP10
• Cleavage: EK (Enterokinase) Recognition Site
• Expression System: E. coli
• Expression Mechanism: Cell-Based Expression
• Protein Tag: His Tag (6x), Xpress Epitope Tag
• Cloning Method: TOPO-TA
• Quantity: 20 reactions
• Vector: pTrc
• Product Line: TOPO™

For Research Use Only. Not for use in diagnostic procedures.