Invitrogen™ PureLink™ Genomic Plant DNA Purification Kit

Catalog No. K183001

Supplier: Invitrogen™ K183001

Description

The PureLink Genomic Plant DNA Purification Kit allows total DNA purification from a variety of plant samples in under one hour.

The PureLink Genomic Plant DNA Purification Kit allows total DNA purification from a variety of plant samples such as spinach, tomato, soy, wheat, Arabidopsis, mushroom, alfalfa sprouts, sunflower sprouts, corn husks, soybeans, mushroom, tomato leaves, wheat grass, and Arabidopsis thaliana leaves in under one hour.

• Efficient—Yields high quality DNA with minimal RNA contamination
• Simple DNA Purification from Plant Samples
• PureLink Genomic Plant DNA Purification Kit is suitable for isolating DNA from chloroplasts and variety of plant tissues
• Entire protocol relies on centrifugation of spin columns in absence of organic solvents
• PureLink Genomic Plant DNA Purification Kit is designed to purify DNA from up to 100 mg of plant tissue

Maximize Yields of Total DNA

• High yield of DNA and minimal DNA degradation is achieved by using young plant samples such as leaves and by freezing sample in liquid nitrogen immediately after collection
• Total DNA is eluted in two aliquots of 100μL each to obtain greater DNA yield, with first elution generating between 65-80% DNA recovery and the second elution > 90%
• Typically, DNA isolated using the PureLink Genomic Plant DNA Purification Kit has an A₂₆₀/A₂₈₀ > 1.80 when samples are diluted in Tris-HCl (pH 7.5), indicating that the DNA is reasonably free of proteins that could interfere with downstream applications

Isolated DNA can be used for a Number of Downstream Applications:

• Total DNA purification from different plant tissue with the PureLink Genomic Plant DNA Purification Kit is suitable for a variety of applications, including PCR, restriction enzyme digestion, and southern blotting

Specifications

• Column Type: Spin Column
• Content And Storage: • 12.5 mL Resuspension Buffer R2; room temperature
  • 5 mL Precipitation Buffer N2; room temperature
  • 7.5 mL Binding Buffer B4; room temperature
  • 25 mL Wash Buffer W4; room temperature
  • 10 mL Wash Buffer W5; room temperature
  • 10 mL Elution Buffer; room temperature
  • 0.75 mL 20% SDS; room temperature
  • 0.75 mL RNase A; room temperature
  • 50 PureLink Spin Columns with Collection Tubes; room temperature
  • 50 Collection Tubes; room temperature
  
  
• Isolation Technology: Silica Spin Column
• Scale: Mini
• Sample Type: Plants
• Elution Volume: 100 to 200 μL
• Final Product Type: gDNA
• For Use With (Application): PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Quantity: 50 Preps
• Shipping Condition: Room Temperature
• Test Time: 40 min.
• Yield: Up to 14.6 μg
• Starting Material Amount: 100 mg

For Research Use Only. Not for use in diagnostic procedures.