Invitrogen™ PureLink™ Genomic Wash Buffer I

Description

Includes

PureLink Genomic Spin Columns, Collection Tubes, Digestion Buffer, Lysis/Binding Buffer, Wash Buffers, Elution Buffer, Proteinase K, RNase A, and the manual. The PureLink 96 Genomic DNA Kit includes PureLink Genomic Binding Plates, Wash Plates, Deep Well Plates, Foil Tape, Digestion Buffer, Lysis/Binding Buffer, Wash Buffers, Elution Buffer, Proteinase K, RNase A, and the manual.

PureLink Genomic DNA Mini Kit and PureLink 96 Genomic DNA Kit enable high-yield, high-purity DNA extractions from wide variety of sample types, including blood, tissues, cells, bacteria, swabs, and blood spots, in a familiar silica spin-column or semi-skirted 96-well plate format.

PureLink Genomic DNA Mini Kit and PureLink 96 Genomic DNA Kit enable high-yield, high-purity DNA extractions from wide variety of sample types, including blood, tissues, cells, bacteria, swabs, and blood spots, in a familiar silica spin-column or semi-skirted 96-well plate format.

• Obtain higher gDNA yields and purity—optimized spin-column membrane and buffer formulations
• Save time and money—use with large range of sample sizes (e.g., 100 μL to 1 mL blood); 96-well version does not require special equipment
• Increase flexibility—one kit works with variety of sample types

How it works

• PureLink Genomic DNA Mini Kit protocol improves upon easy, familiar spin-column methods currently in use
• Columns are newly configured to bind and release DNA more efficiently
• Samples are lysed in optimized buffer formulated to enhance proteinase K activity and eliminate protein contamination
• Powerful wash buffers remove all traces of protein and salt
• DNA is eluted in low-salt buffer to allow for pH stabilization of the DNA in storage

For higher throughput, use the PureLink 96 Genomic DNA Kit

• In this 96-well version, sample processing is performed with either vacuum manifold or centrifugation at only 2,250 g, allowing more flexibility in centrifuge choice
• The semi-skirted plate design is compatible with most vacuum manifolds on robotic workstations

Higher yields and purity

• With the PureLink Genomic DNA Kits, get greater yields of higher-purity gDNA (determined by A260/A280 measurements) with all sample types - from bacteria to tissues to blood to cells
• PureLink 96 Genomic DNA Kit outperforms competitor products in both DNA yield and purity for multiple sample types, including blood
• A260/A280 and A260/A230 ratios of PureLink gDNA are higher than those obtained with other kits, indicating no contamination carryover of protein or guanidine using the enhanced PureLink Lysis and Wash Buffers

Order Info

Shipping Condition: Room Temperature

Specifications

• Buffer: Wash Buffer
• Column Type: Spin Column
• Content And Storage: Store at room temperature.
• Format: Kit
• Sample Type: Bacteria, Blood, Blood Spot, Cells, Swab, Tissue
• Product Line: PureLink™
• Product Type: Genomic Wash Buffer I
• Quantity: 100 mL
• Shipping Condition: Room Temperature

For Research Use Only. Not for use in diagnostic procedures.