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Invitrogen™ PureLink™ Viral RNA/DNA Mini Kit
Description
Includes
- 32mL Viral Lysis buffer (L22)
- 15mL 5X Wash Buffer (W5)
- 2 x 1.6mL Proteinase K (20mg/mL in storage buffer)
- 310μg lyophilized Carrier RNA
- 15.5mL Sterile, RNAase-free water
- 50 Viral Spin Columns with Collection Tubes
- 2 x 50 Wash Tubes (2.0mL)
- 50 Recovery Tubes (1.5mL)
The PureLink Viral RNA/DNA Mini Kit provides a rapid and efficient method to simultaneously purify viral RNA/DNA from fresh or frozen cell-free biological fluids (plasma, serum, cerebrospinal fluid) and cell culture supernatants. The kit is specifically designed to isolate high-quality viral nucleic acids from a variety of RNA and DNA viruses within 45 minutes using low elution volumes that allow sensitive downstream analysis. The purified viral RNA/DNA is devoid of proteins and nucleases and is suitable for use in downstream applications that allow viral detection and genotyping.
The PureLink Viral Mini Kit has been used to purify viral dsDNA from orthopoxviruses including cowpox virus, variola virus, monkeypox virus, vaccinia virus, and HERV.
The PureLink Viral RNA/DNA Mini Kit provides the following advantages:
• Rapid and efficient purification of high-quality viral nucleic acid using spin column-based centrifugation with no sample cross-contamination
• Specifically designed to purify viral RNA and DNA from ≤500 μL cell-free samples within 45 min
• Ability to elute viral nucleic acids in low elution volumes of 10–50 μL to allow sensitive downstream analysis
Order Info
Shipping Condition: Room Temperature
Specifications
Specifications
| Column Type | Spin Column |
| Content And Storage | • 310 μg Carrier RNA; –20°C • 32 mL Viral Lysis Buffer L22; room temperature • 15 mL Wash Buffer WII; room temperature • 2 x 1.6 mL Proteinase K; room temperature, or 4°C for long-term storage • 15.5 mL RNase-free Water E3; room temperature • 50 Viral Spin Columns with Collection Tubes; room temperature • 2 x 50 Wash Tubes; room temperature • 50 Recovery Tubes; room temperature |
| Isolation Technology | Silica Spin Column |
| Scale | Mini |
| Sample Type | Cells, Cerebrospinal Fluid, Plasma, Serum, Cell Culture Supernatants, Raw and Stabilized Saliva, Nasopharyngeal Viral Transfer Medium (NPVTM) |
| Elution Volume | 10 to 50 μL |
| Final Product Type | Viral RNA and DNA |
| For Use With (Application) | RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| Quantity | 50 Preps |
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Frequently Asked Questions (FAQs)
Yes, the Cat. No. for 100 wash tubes is 12282-100.
The Proteinase K solution is stable for 1 year when stored at room temperature. For long-term storage (>1 year) or if room temperature is >25 degrees C, store the Proteinase K solution at 4 degrees C.
Plasma is the liquid portion of the blood that is separated from the blood cells by centrifugation. One of the characteristics of plasma is that it clots easily, which is important for hemophiliacs needing a transfusion but is a nuisance in most other applications. By agitating the plasma, one can precipitate the clotting factors as a large clot, and the leftover fluid is called serum. In other words, serum plus clotting factors is plasma, and clotted plasma yields serum.
The tRNA is stable for over one year. There was no significant difference in qRT-PCR results with tRNA stored for 10 days at ‘20 degrees C or 42 degrees C. The lyophilized tRNA is stable at ‘20 degrees C, room temperature, 37 degrees C, and 45 degrees C for up to 1 month.
The carrier RNA is tRNA and it may not be necessary. We have tested without the tRNA and obtained excellent results. The tRNA provides a substrate for RNases in the sample to protect the viral RNA until the virus is lysed. You may want to scale down the amount of carrier RNA or leave it out. However, you should validate it in your application to make sure you get the same results as when you include it.
Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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