Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Invitrogen™ PureLink™ Viral RNA/DNA Mini Kit

Catalog No. 12280050
Encompass_Preferred
Click to view available options
Quantity:
50 Preps

Includes

  • 32mL Viral Lysis buffer (L22)
  • 15mL 5X Wash Buffer (W5)
  • 2 x 1.6mL Proteinase K (20mg/mL in storage buffer)
  • 310μg lyophilized Carrier RNA
  • 15.5mL Sterile, RNAase-free water
  • 50 Viral Spin Columns with Collection Tubes
  • 2 x 50 Wash Tubes (2.0mL)
  • 50 Recovery Tubes (1.5mL)

The PureLink Viral RNA/DNA Mini Kit provides a rapid and efficient method to simultaneously purify viral RNA/DNA from fresh or frozen cell-free biological fluids (plasma, serum, cerebrospinal fluid) and cell culture supernatants.

The PureLink Viral RNA/DNA Mini Kit provides a rapid and efficient method to simultaneously purify viral RNA/DNA from fresh or frozen cell-free biological fluids (plasma, serum, cerebrospinal fluid) and cell culture supernatants. The kit is specifically designed to isolate high-quality viral nucleic acids from a variety of RNA and DNA viruses within 45 minutes using low elution volumes that allow sensitive downstream analysis. The purified viral RNA/DNA is devoid of proteins and nucleases and is suitable for use in downstream applications that allow viral detection and genotyping.

The PureLink Viral Mini Kit has been used to purify viral dsDNA from orthopoxviruses including cowpox virus, variola virus, monkeypox virus, vaccinia virus, and HERV.

The PureLink Viral RNA/DNA Mini Kit provides the following advantages:
• Rapid and efficient purification of high-quality viral nucleic acid using spin column-based centrifugation with no sample cross-contamination
• Specifically designed to purify viral RNA and DNA from ≤500 μL cell-free samples within 45 min
• Ability to elute viral nucleic acids in low elution volumes of 10–50 μL to allow sensitive downstream analysis

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Specifications

Column Type Spin Column
Content And Storage • 310 μg Carrier RNA; –20°C
• 32 mL Viral Lysis Buffer L22; room temperature
• 15 mL Wash Buffer WII; room temperature
• 2 x 1.6 mL Proteinase K; room temperature, or 4°C for long-term storage
• 15.5 mL RNase-free Water E3; room temperature
• 50 Viral Spin Columns with Collection Tubes; room temperature
• 2 x 50 Wash Tubes; room temperature
• 50 Recovery Tubes; room temperature

Isolation Technology Silica Spin Column
Scale Mini
Sample Type Cells, Cerebrospinal Fluid, Plasma, Serum, Cell Culture Supernatants, Raw and Stabilized Saliva, Nasopharyngeal Viral Transfer Medium (NPVTM)
Elution Volume 10 to 50 μL
Final Product Type Viral RNA and DNA
For Use With (Application) RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization
High-throughput Compatibility Not High-throughput Compatible (Manual)
Quantity 50 Preps
Shipping Condition Room Temperature
Test Time 45 min.
Yield 5 μg (Binding capacity)
Starting Material Amount ≤500 μL
Show More Show Less
WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

Product Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Your feedback has been submitted: Thank you for helping us improve our website.