Invitrogen™ PureLink™ Viral RNA/DNA Mini Kit

Provides a rapid and efficient method to simultaneously purify viral RNA/DNA from fresh or frozen cell-free biological fluids (plasma, serum, cerebrospinal fluid) and cell culture supernatants.

Manufacturer:  Invitrogen™ 12280050

Catalog No. 12-280-050

Includes:

• 32mL Viral Lysis buffer (L22)
• 15mL 5X Wash Buffer (W5)
• 2 x 1.6mL Proteinase K (20mg/mL in storage buffer)
• 310μg lyophilized Carrier RNA
• 15.5mL Sterile, RNAase-free water
• 50 Viral Spin Columns with Collection Tubes
• 2 x 50 Wash Tubes (2.0mL)
• 50 Recovery Tubes (1.5mL)

Description

• Kit provides a rapid and efficient method to simultaneously purify viral RNA/DNA from fresh or frozen cell-free biological fluids (plasma, serum, cerebrospinal fluid) and cell culture supernatants
• Specifically designed to isolate high-quality viral nucleic acids from a variety of RNA and DNA viruses within 45 minutes using low elution volumes that allow sensitive downstream analysis
• The purified viral RNA/DNA is devoid of proteins and nucleases and suitable for use in downstream applications that allow viral detection and genotyping
• The PureLink Viral Mini Kit has been used to purify viral dsDNA from orthopoxviruses including cowpox virus, variola virus, monkeypox virus, vaccinia virus, and HERV
• Rapid and efficient purification of high-quality viral nucleic acid using spin column-based centrifugation with no sample cross-contamination
• Specifically designed to purify viral RNA and DNA from ≤ 500 µL cell-free samples within 45 minutes
• Capable of eluting viral nucleic acids in low elution volumes of 10–50 µL to allow sensitive downstream analysis

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Order Info

Shipping Condition: Room Temperature

Specifications

• Column Type: Spin Column
• Format: Kit
• Sample Type: Cell-Free Fluids, Cerebrospinal Fluid, Liquid Samples (e.g. Serum), Plasma, Serum, Virus
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Product Line: PureLink™
• Quantity: 50 Preps
• Target: Viral DNA⁄RNA

• Content And Storage: The PureLink™ Viral RNA/DNA Mini Kit contains enough reagents for 50 reactions.
  
  Kit Contents:
  • 32 ml Viral Lysis buffer (L22)
  • 15 ml 5X Wash Buffer (W5)
  • 2 × 1.6 ml Proteinase K (20 mg/ml in storage buffer)
  • 310 µg lyophilized Carrier RNA
  • 15.5 ml Sterile, RNAase-free water
  • 50 Viral Spin Columns with Collection Tubes
  • 2 × 50 Wash Tubes (2.0 ml)
  • 50 Recovery Tubes (1.5 ml)
  
  Store all components except for Carrier RNA at room temperature. Carrier RNA should be stored at −20°C.
• Isolation Technology: Silica Spin Column
• For Use With (Application): Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
• No. of Reactions: 50 Preps
• Product Type: Viral RNA/DNA Mini Kit
• Shipping Condition: Room Temperature
• Test Time: 45 min

For Research Use Only. Not for use in diagnostic procedures.