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Invitrogen™ RNaseOUT™ Recombinant Ribonuclease Inhibitor

Catalog No. 10777019
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Quantity:
5000 units

Protects mRNA and improves total cDNA yields

RNaseOUT Recombinant Ribonuclease Inhibitor is a potent noncompetitive inhibitor of pancreatic-type ribonucleases such as RNase A and is used to avoid RNA degradation in a variety of applications. It is an acidic protein with a molecular weight of ∼52 kDa that inhibits RNase A, RNase B, and RNase C.

The robust molecular structure of the RNaseOUT™ inhibitor together with a very high binding affinity for pancreatic-type RNases ensures superior protection of RNA from degradation making RNaseOUT™ highly effective and reliable in various molecular biology applications. 

Features

  • Stable under prolonged reaction conditions—effective RNA protection from RNases at:
    • 4°C for at least 96 hours
    • 25°C for at least 96 hours
    • 37°C for 24 hours
    • 50°C for up to 6 hours
  • Oxidation resistant—highly stable and requires low concentrations of DTT to retain RNase inhibiting activity
  • Wide activity temperature range—maintains full activity from 4°C to 55°C
  • Superior quality—stringent quality control and industry leading manufacturing process

Applications

  • In vitro transcription and translation
  • CRISPR guide RNA production
  • RNA purification
  • cDNA synthesis
  • RT-PCR
  • RT-LAMP
  • RNA-seq
  • RNA modification and labeling

Source

Purified by affinity chromatography from E. coli expressing a cloned porcine gene.

Performance and quality testing

  • SDS-PAGE purity
  • Endo-deoxyribonuclease assay
  • Protein concentration
  • Specific activity
  • Performance evaluated by RT-PCR

Unit definition

One unit inhibits 5 ng of RNase A by 50% using cytidine 2', 3' cyclic monophosphate (cCMP) as the substrate.

Unit reaction conditions

100 mM Tris-acetate (pH 6.5), 1 mM EDTA, 0.2 mM cCMP, 2 μg RNase A in 1 mL from 0 to 10 min at 25°C

Order Info

Shipping Condition: Dry ice

TRUSTED_SUSTAINABILITY

Specifications

Concentration 40 U/μL
Content And Storage Store at -20°C. Avoid exposing product to frequent changes in temperature. RNaseOUT™ Ribonuclease Inhibitor requires 1 mM DTT to maintain activity.

The product is guaranteed for 6 months from date of purchase unless otherwise stated in the product documentation.
Purification Time 10 min.
Product Line RNaseOUT™
Quantity 5000 units
Shipping Condition Dry Ice
WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

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