Invitrogen™ RNaseOUT™ Recombinant Ribonuclease Inhibitor  

Protects mRNA and improves total cDNA yields

Manufacturer:  Invitrogen™ 10777019

Catalog No. 10-777-019

Description

RNaseOUT Recombinant Ribonuclease Inhibitor is a potent noncompetitive inhibitor of pancreatic-type ribonucleases such as RNase A, and is used to avoid RNA degradation in a variety of applications. RNaseOUT Recombinant Ribonuclease Inhibitor is an acidic protein with a molecular weight of approx. 52 kDa. RNaseOUT inhibits RNase A, RNase B, and RNase C.
Applications cDNA synthesis, RT-PCR, and in vitro transcription and translation
Source Purified by affinity chromatography from E. coli expressing a cloned porcine gene
Performance and quality testing SDS-PAGE purity, endodeoxyribonuclease assay, protein concentration, specific activity, performance evaluated by RT-PCR
Unit definition One unit inhibits 5 ng of RNase A by 50% using cytidine 2', 3' cyclic monophosphate (cCMP) as the substrate
Unit reaction conditions 100mM Tris-acetate (pH 6.5), 1mM EDTA, 0.2mM cCMP, 2 µg RNase A in 1mL from 0 to 10 min at 25°C

PCR and Real-Time PCR, Reverse Transcription

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Order Info

Shipping Condition: Dry ice

Specifications

• Quantity: 5000 units

For Research Use Only. Not for use in diagnostic procedures.