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Invitrogen™ RNaseOUT™ Recombinant Ribonuclease Inhibitor
Description
RNaseOUT Recombinant Ribonuclease Inhibitor is a potent noncompetitive inhibitor of pancreatic-type ribonucleases such as RNase A and is used to avoid RNA degradation in a variety of applications. It is an acidic protein with a molecular weight of ∼52 kDa that inhibits RNase A, RNase B, and RNase C.
The robust molecular structure of the RNaseOUT™ inhibitor together with a very high binding affinity for pancreatic-type RNases ensures superior protection of RNA from degradation making RNaseOUT™ highly effective and reliable in various molecular biology applications.
Features
- Stable under prolonged reaction conditions—effective RNA protection from RNases at:
- 4°C for at least 96 hours
- 25°C for at least 96 hours
- 37°C for 24 hours
- 50°C for up to 6 hours
- Oxidation resistant—highly stable and requires low concentrations of DTT to retain RNase inhibiting activity
- Wide activity temperature range—maintains full activity from 4°C to 55°C
- Superior quality—stringent quality control and industry leading manufacturing process
Applications
- In vitro transcription and translation
- CRISPR guide RNA production
- RNA purification
- cDNA synthesis
- RT-PCR
- RT-LAMP
- RNA-seq
- RNA modification and labeling
Source
Purified by affinity chromatography from E. coli expressing a cloned porcine gene.
Performance and quality testing
- SDS-PAGE purity
- Endo-deoxyribonuclease assay
- Protein concentration
- Specific activity
- Performance evaluated by RT-PCR
Unit definition
One unit inhibits 5 ng of RNase A by 50% using cytidine 2', 3' cyclic monophosphate (cCMP) as the substrate.
Unit reaction conditions
100 mM Tris-acetate (pH 6.5), 1 mM EDTA, 0.2 mM cCMP, 2 μg RNase A in 1 mL from 0 to 10 min at 25°C
Specifications
Specifications
| Concentration | 40 U/μL |
| Content And Storage | Store at -20°C. Avoid exposing product to frequent changes in temperature. RNaseOUT™ Ribonuclease Inhibitor requires 1 mM DTT to maintain activity. The product is guaranteed for 6 months from date of purchase unless otherwise stated in the product documentation. |
| Product Line | RNaseOUT |
| Quantity | 5000 Units |
Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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