Invitrogen™ S.N.A.P.™ Gel Purification Kit

Catalog No.	Quantity
K199925	25 preps

Catalog No. K199925 Supplier Invitrogen™ Supplier No. K199925

Description

Includes

Each S.N.A.P. Gel Purification Kit includes enough S.N.A.P. columns, collection vials, and reagents for 25 gel purifications.

The S.N.A.P.™ Gel Purification Kit is designed for rapid purification of DNA fragments from agarose gels. The key is unique S.N.A.P. resin, which is provided ready to use in a spin-column format.

The S.N.A.P.™ Gel Purification Kit is designed for rapid purification of DNA fragments from agarose gels. The key is unique S.N.A.P. resin, which is provided ready to use in a spin-column format. The resin efficiently binds nucleic acids up to 20 kb and eliminates organic extractions, and need to perform time-consuming ethanol precipitations.

• S.N.A.P.™ Gel Purification Kit yields pure DNA in an easy-to-perform, rapid procedure
• Excise DNA band from your agarose gel
• Add NaI solution (provided) and melt the agarose at 42–50°C
• Load sample onto the S.N.A.P.™ spin column
• Wash away contaminants
• Elute DNA in water

Specifications

• Column Type: Spin Column
• Content And Storage: Each S.N.A.P.™ Gel Purification Kit includes enough S.N.A.P.™ columns, collection vials, and reagents for 25 gel purifications. Store all components at room temperature. All reagents are guaranteed stable for 6 months when properly stored.
• Format: Kit
• Isolation Technology: Spin Column
• Sample Type: Gel
• For Use With (Application): Cloning, PCR
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Label or Dye: Ethidium Bromide, SYBR Safe
• No. of Reactions: 25 Preps
• Product Line: S.N.A.P.
• Product Type: Gel Purification Kit
• Quantity: 25 preps
• Target: Nucleic Acids in Gel Fragments

Frequently Asked Questions (FAQs)

I'm running an agarose gel with TAE or TBE. Can I use your gel extraction kits?

Yes, both TAE and TBE agarose gels are compatible with our gel extraction kits.

What is the difference between Purelink PureLink Quick Gel Extraction System and S.N.A.P. Gel Purification Kit?

The PureLink kit can be used with both TAE and TBE gels, while the S.N.A.P. Gel Purification Kit is recommended for TAE gels only.

Can you use TBE gels with S.N.A.P. gel purification kits?

No, TBE gels contain borate, which interferes with the sodium iodide solution. Therefore, only TAE gels can be used. We recommend the PureLink Quick Gel Extraction kit for both TAE and TBE agarose gels.

What is the smallest fragment that can be purified with S.N.A.P. columns?

S.N.A.P. mini prep columns have been used to purify fragments of 100 bp in gel purification procedures.

What is the resin in the S.N.A.P. kits?

The column contains a silica/glass fiber based resin. However, the exact composition is proprietary.

What is unique about the S.N.A.P. UV-free Gel Purification Kit?

The S.N.A.P. UV-free Gel Purification Kit utilizes crystal violet as a staining reagent, which can be visualized in non-UV light conditions. This can prevent DNA damage that can be caused by the UV light required by normal ethidium bromide staining. DNA fragments recovered using this kit have higher cloning efficiency. In addition, DNA can be visualized under normal light as a thin violet band while the gel is running and excised as soon as bands are sufficiently resolved.

For Research Use Only. Not for use in diagnostic procedures.