Invitrogen™ S.N.A.P.™ Plasmid DNA MidiPrep Kit

Catalog No.	Quantity
K191001	20 preps

Catalog No. K191001 Supplier Invitrogen™ Supplier No. K191001

Description

Includes

S.N.A.P. MidiPrep filtering columns, S.N.A.P. MidiPrep DNA binding columns, lysis buffer, binding buffer, wash buffer, 4X final wash, resuspension buffer, precipitation salt, and RNase A

The S.N.A.P.™ MidiPrep Kit is medium-scale plasmid DNA purification kit designed for fast and easy purification of pure plasmid DNA from 10–100 mL of overnight culture.

The S.N.A.P.™ MidiPrep Kit is medium-scale plasmid DNA purification kit designed for fast and easy purification of pure plasmid DNA from 10–100 mL of overnight culture (Figure 1). The S.N.A.P. MidiPrep procedure takes just 40 minutes.

• Lyse cells and filter debris with the S.N.A.P. filtering column
• Apply flow-through to the S.N.A.P.™ DNA binding column
• Wash the column
• Elute pure, supercoiled plasmid DNA in water or TE buffer

Specifications

• Content And Storage: Store the S.N.A.P.™ MidiPrep filtering columns, S.N.A.P.™ MidiPrep DNA binding columns, lysis buffer, binding buffer, wash buffer, and 4X final wash at room temperature. Store the resuspension buffer, precipitation salt, and RNase A at +4°C. All reagents are guaranteed stable for 6 months when properly stored.
• Format: Kit
• Isolation Technology: Spin Column
• Scale: Midi
• Sample Type: Bacterial Culture
• Final Product Type: Plasmid DNA
• For Use With (Application): Sequencing, Cloning, Transformation, Transfection, In Vitro Transcription
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Prep Scale: 100-200 μg (Medium-Scale) Plasmid DNA
• Product Line: S.N.A.P.
• Product Type: Plasmid DNA MidiPrep Kit
• Quantity: 20 preps
• Target: Plasmid DNA
• Test Time: 40 min.

Frequently Asked Questions (FAQs)

Which centrifuge is used at Thermo Fisher Scientific for the S.N.A.P. MidiPrep protocol?

The Sorval RT6000B table-top centrifuge is used at Thermo Fisher Scientific for the S.N.A.P. MidiPrep protocol. The low-speed spins are around 2,000 rpm and the high-speed spins are around 3,000 rpm. You may also use the Sorval RC-5B centrifuge with the SS-34 rotor. However, you might observe some spillage with this centrifuge.

What is the upper size limit for plasmid purification with the S.N.A.P. Miniprep Kit?

Bacmid DNA (>100 kbp) has been purified with the S.N.A.P. miniprep kit.

The PureLink HiPure Plasmid DNA Purification Kits (mini/midi/maxi scales) can also be used for large constructs. The HiPure kit manuals contain a modified protocol designed to help increase yields with these large plasmid isolations.

What are the compositions of the S.N.A.P. MiniPrep and MidiPrep Kit solutions?

The formulations are published in the manual for the S.N.A.P. MidiPrep kit, and are the same as the ones used in the MiniPrep kit.

What is the resin in the S.N.A.P. kits?

The column contains a silica/glass fiber based resin. However, the exact composition is proprietary.

Why is LB medium recommended for growing up plasmid preps in the S.N.A.P. protocol?

The volumes of culture recommended in our S.N.A.P. kits assume an OD of no more than 2.0. If a rich medium is used, the volume processed should be reduced to avoid overloading the column.

Can I use a clinical centrifuge for the S.N.A.P. midiprep and midiprep systems?

No, we know of no clinical centrifuges that can reach 5,000 x g. Most clinical centrifuges have a maximum in the range of 3,000 to 4,000 x g. A high-speed swinging bucket rotor is therefore required for these systems. Often researchers will attempt to compensate for lower g force by increasing centrifugation time; however, this will not work.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.