Invitrogen™ S.N.A.P.™ Plasmid DNA MiniPrep Kit

Catalog No.	Quantity
K190001	100 preps

Catalog No. K190001 Supplier Invitrogen™ Supplier No. K190001

Description

Includes

S.N.A.P. MidiPrep filtering columns, S.N.A.P. MidiPrep DNA binding columns, lysis buffer, binding buffer, wash buffer, 4X final wash, resuspension buffer, precipitation salt, and RNase A

The S.N.A.P.™ MiniPrep Kit is plasmid DNA purification system designed to yield pure plasmid DNA for automated sequencing in just 25 minutes.

The S.N.A.P.™ MiniPrep Kit is plasmid DNA purification system designed to yield pure plasmid DNA (Figures 1 and 2) for automated sequencing in just 25 minutes

• DNA can improve your automated sequencing results by producing:
• Longer reads
• Lower background
• Higher accuracy
• Stronger signal peaks

Specifications

• Content And Storage: Store the S.N.A.P.™ MiniPrep spin columns, lysis buffer, binding buffer, wash buffer, 4X final wash, and the collection tubes at room temperature. Store the resuspension buffer, precipitation salt solution, and RNase A at +4°C. All reagents are guaranteed stable for 6 months when properly stored.
• Format: Kit
• Isolation Technology: Spin Column
• Sample Type: Bacterial Culture
• Final Product Type: Plasmid DNA
• For Use With (Application): Next-Generation Sequencing, In Vitro Transcription, Sequencing, Cloning, Transformation
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• No. of Reactions: 100 preps
• Prep Scale: < 100 μg (Small-Scale) Plasmid DNA
• Product Line: S.N.A.P.
• Product Type: Plasmid DNA MiniPrep Kit
• Quantity: 100 preps
• Target: Plasmid DNA
• Test Time: 25 min.

Frequently Asked Questions (FAQs)

What is the upper size limit for plasmid purification with the S.N.A.P. Miniprep Kit?

Bacmid DNA (>100 kbp) has been purified with the S.N.A.P. miniprep kit.

The PureLink HiPure Plasmid DNA Purification Kits (mini/midi/maxi scales) can also be used for large constructs. The HiPure kit manuals contain a modified protocol designed to help increase yields with these large plasmid isolations.

What are the compositions of the S.N.A.P. MiniPrep and MidiPrep Kit solutions?

The formulations are published in the manual for the S.N.A.P. MidiPrep kit, and are the same as the ones used in the MiniPrep kit.

What is the resin in the S.N.A.P. kits?

The column contains a silica/glass fiber based resin. However, the exact composition is proprietary.

Why is LB medium recommended for growing up plasmid preps in the S.N.A.P. protocol?

The volumes of culture recommended in our S.N.A.P. kits assume an OD of no more than 2.0. If a rich medium is used, the volume processed should be reduced to avoid overloading the column.

Why can't I dissolve all of my DNA after precipitation?

Sometimes there is insoluble material (probably resin fines) in the precipitated DNA. This material is very easily removed with a 10 second spin. The presence of these particles does not reflect a poor preparation. Once the particles are removed, the DNA performs well in all applications.

What products does Thermo Fisher Scientific sell for plasmid purification?

Please visit our Plasmid DNA Purification page for the most up-to-date information:
http://www.thermofisher.com/us/en/home/life-science/DNA-RNA-Purification-Analysis/DNA-Purification/Plasmid-DNA-Purification.html

Can I use a clinical centrifuge for the S.N.A.P. midiprep and midiprep systems?

No, we know of no clinical centrifuges that can reach 5,000 x g. Most clinical centrifuges have a maximum in the range of 3,000 to 4,000 x g. A high-speed swinging bucket rotor is therefore required for these systems. Often researchers will attempt to compensate for lower g force by increasing centrifugation time; however, this will not work.

What systems does Thermo Fisher Scientific offer for plasmid DNA Miniprep and Midipreps?

We have many products suitable for Miniprep and Midiprep purifications:

The PureLink HiPure Plasmid Miniprep and Midiprep purification kits are based on anion-exchange technology and allow high-yield isolation of highly pure plasmid DNA, equivalent to two passes through CsCl (cesium chloride) gradients. Plasmid isolated with these kits will have the lowest endotoxin levels and will work best for difficult transfections and other challenging applications.

The PureLink HQ Mini Plasmid DNA Purification Kit is a modified silica technology designed to yield high amounts of high-quality plasmid DNA for use in standard mammalian cell transfections, restriction enzyme digestion, PCR, automated sequencing, and transformation. This kit is also available in 96-well format.

The PureLink Quick Plasmid Miniprep Kit is a silica-based purification kit designed to isolate high-quality plasmid DNA suitable for sequencing and cloning applications in 25-45 minutes.

The ChargeSwitch-Pro Plasmid Miniprep Kit uses our ChargeSwitch technology where plasmid is bound to a surface ligand that is positively charged at low pH, and eluted when the pH is raised to 8.5. The ChargeSwitch protocol retains the ease and familiarity of common silica spin-column protocols with binding, washing, and elution steps. Since this method does not require the use of ethanol, chaotropic salts, or organic solvents for nucleic acid purification, the resulting DNA is free of potentially inhibitory compounds.

The ChargeSwitch Plasmid ER and Plasmid Yeast Mini kits use our ChargeSwitch magnetic bead-based technology for isolation of high-quality plasmid DNA.

The ChargeSwitch NoSpin Plasmid Micro kit is a novel magnetic bead technology that eliminates the need for centrifugation in the plasmid purification procedures.

The ChargeSwitch products are easily automated on robotic platforms.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.