Invitrogen™ S.N.A.P.™ UV-Free Gel Purification Kit

Catalog No.	Quantity
K200025	25 preps

Catalog No. K200025 Supplier Invitrogen™ Supplier No. K200025

Description

Includes

Each S.N.A.P. UV-Free Gel Purification Kit includes enough crystal violet reagents, S.N.A.P. columns, collection vials, sodium iodide solution, purification buffers, and sterile water for 25 gel purifications.

The S.N.A.P.™ UV-Free Gel Purification Kit uses non-toxic reagents to rapidly purify intact DNA fragments from agarose gels.

The S.N.A.P.™ UV-Free Gel Purification Kit uses non-toxic reagents to rapidly purify intact DNA fragments from agarose gels. The key to isolating undamaged DNA is the unique crystal violet reagent, which allows visualization of DNA (≥200 ng) in the gel under ambient light. This eliminates the need for ethidium bromide and exposure to UV light, which can nick and damage DNA. Nucleic acids up to 7 kb are efficiently purified from the gel using the convenient S.N.A.P.™ resin, provided ready to use in a spin column format. The resin eliminates organic extractions, which generate hazardous waste. In addition, there are no time-consuming ethanol precipitations.

Easy to Use
The S.N.A.P.™ UV-Free Gel Purification Kit yields pure, intact DNA in an easy-to-perform, rapid procedure. Simply:
1. Prepare gel with crystal violet reagent and load samples in crystal violet loading buffer.
2. Visualize DNA band under ambient light and excise from agarose gel.
3. Add NaI solution (provided) and melt the agarose at 42-50°C.
4. Load sample onto the S.N.A.P.™ spin column.
5. Wash away contaminants.
6. Elute DNA in water.

The eluted DNA is highly pure, intact, and ready to use for cloning, sequencing, transformation, or transfection.

Specifications

• Column Type: Spin Column
• Content And Storage: Each S.N.A.P.™ UV-Free Gel Purification Kit includes enough crystal violet reagents, S.N.A.P.™ columns, collection vials, sodium iodide solution, purification buffers, and sterile water for 25 gel purifications. Store all components at room temperature. All reagents are guaranteed stable for 6 months when properly stored.
• Format: Kit
• Isolation Technology: Spin Column
• Sample Type: DNA Fragments in Agarose Gel Slices, Gel Samples
• For Use With (Application): Cloning, PCR
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Label or Dye: Crystal Violet
• Product Line: S.N.A.P.
• Product Type: Gel Purification Kit
• Quantity: 25 preps
• Target: Nucleic Acids in Gel Fragments

Frequently Asked Questions (FAQs)

I'm running an agarose gel with TAE or TBE. Can I use your gel extraction kits?

Yes, both TAE and TBE agarose gels are compatible with our gel extraction kits.

Can you use TBE gels with S.N.A.P. gel purification kits?

No, TBE gels contain borate, which interferes with the sodium iodide solution. Therefore, only TAE gels can be used. We recommend the PureLink Quick Gel Extraction kit for both TAE and TBE agarose gels.

What is the smallest fragment that can be purified with S.N.A.P. columns?

S.N.A.P. mini prep columns have been used to purify fragments of 100 bp in gel purification procedures.

What is the resin in the S.N.A.P. kits?

The column contains a silica/glass fiber based resin. However, the exact composition is proprietary.

What is unique about the S.N.A.P. UV-free Gel Purification Kit?

The S.N.A.P. UV-free Gel Purification Kit utilizes crystal violet as a staining reagent, which can be visualized in non-UV light conditions. This can prevent DNA damage that can be caused by the UV light required by normal ethidium bromide staining. DNA fragments recovered using this kit have higher cloning efficiency. In addition, DNA can be visualized under normal light as a thin violet band while the gel is running and excised as soon as bands are sufficiently resolved.

For Research Use Only. Not for use in diagnostic procedures.