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Invitrogen™ SuperScript™ One-Cycle cDNA Kit for use with Affymetrix™ One-Cycle Assays Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Conveniently contains all of the reagents required for double-stranded cDNA synthesis within the Affymetrix GeneChip™ workflow

Manufacturer:  Invitrogen™ A10752030

Catalog No. A10752030


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Includes: 50µM T7-Oligo(dT) Primer, 5X 1st Strand Reaction Mix, 0.1M DTT, 10mM dNTP, SuperScript™ II (200u/µL), 5X 2nd Strand Reaction Mix, E.Coli DNA Ligase, E.Coli DNA Polymerase I, RNase H, T4 DNA Polymerase, 0.5M EDTA, RNase-free Water

Description

Description

The kit includes Invitrogen's proprietary RNase H reduced activity SuperScript™ II Reverse Transcriptase and a qualified oligo (dt)-containing primer to perform reactions standard to Affymetrix– protocol. Designed to consistently produce higher yields of full-length cDNA for Affymetrix One-Cycle assays using either total RNA or poly A+ selected RNA (mRNA).

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Shipping Condition: Dry Ice

Specifications

Specifications

Biotin-Based
For generating double-stranded cDNA
50μM T7-Oligo(dT) Primer, 5X 1st Strand Reaction Mix, 0.1M DTT, 10mM dNTP, SuperScript™ II (200u/μL), 5X 2nd Strand Reaction Mix, E.Coli DNA Ligase, E.Coli DNA Polymerase I, Rnase H, T4 DNA Polymerase, 0.5M EDTA, Rnase-free Water
30 reactions
Store at −20°C
cDNA (Double-Stranded)
Affymetrix GeneChip™
Biotin
Total RNA, Poly(A+) RNA
cDNA
Documents
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For Research Use Only. Not for use in diagnostic procedures.