Invitrogen SuperScript One-Cycle cDNA Kit for use with Affymetrix One-Cycle Assays
Conveniently contains all of the reagents required for double-stranded cDNA synthesis within the Affymetrix GeneChip workflow
Manufacturer: Invitrogen A10752030
The kit includes Invitrogen's proprietary RNase H reduced activity SuperScript II Reverse Transcriptase and a qualified oligo (dt)-containing primer to perform reactions standard to Affymetrix protocol. Designed to consistently produce higher yields of full-length cDNA for Affymetrix One-Cycle assays using either total RNA or poly A+ selected RNA (mRNA).
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Shipping Condition: Dry Ice
|For generating double-stranded cDNA|
|50μM T7-Oligo(dT) Primer, 5X 1st Strand Reaction Mix, 0.1M DTT, 10mM dNTP, SuperScript™ II (200u/μL), 5X 2nd Strand Reaction Mix, E.Coli DNA Ligase, E.Coli DNA Polymerase I, Rnase H, T4 DNA Polymerase, 0.5M EDTA, Rnase-free Water|
|Store at −20°C|
|Total RNA, Poly(A+) RNA|
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For Research Use Only. Not for use in diagnostic procedures.