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Invitrogen™ SuperScript™ One-Cycle cDNA Kit for use with Thermo Scientific™ One-Cycle Assays
Description
Includes
50μM T7-Oligo(dT) Primer, 5X 1st Strand Reaction Mix, 0.1M DTT, 10mM dNTP, SuperScript™ II (200u/μL), 5X 2nd Strand Reaction Mix, E.Coli DNA Ligase, E.Coli DNA Polymerase I, RNase H, T4 DNA Polymerase, 0.5M EDTA, RNase-free Water
The kit includes Invitrogen's proprietary RNase H reduced activity SuperScript™ II Reverse Transcriptase and a qualified oligo (dt)-containing primer to perform reactions standard to Affymetrix– protocol. Designed to consistently produce higher yields of full-length cDNA for Affymetrix One-Cycle assays using either total RNA or poly A+ selected RNA (mRNA).
Order Info
Shipping Condition: Dry Ice
Specifications
Specifications
| Content And Storage | 50 μM T7-Oligo(dT) Primer, 5X 1st Strand Reaction Mix, 0.1M DTT, 10 mM dNTP, SuperScript™ II (200u⁄ul), 5X 2nd Strand Reaction Mix, E.Coli DNA Ligase, E.Coli DNA Polymerase I, RNase H, T4 DNA Polymerase, 0.5M EDTA, RNase-free Water All components are guaranteed stable for 6 months when properly stored. |
| Detection Method | Biotin-based |
| Format | Kit |
| Label or Dye | Biotin |
| Final Product Type | cDNA (Double-stranded) |
| Sample Type | Poly(A+) RNA, Total RNA |
| Labeling Target | cDNA |
| For Use With (Application) | For generating double-stranded cDNA |
| Includes Label or Dye | No |
| For Use With (Equipment) | Affymetrix GeneChip™ |
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Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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