Invitrogen™ T-REx™ Core Kit, with pcDNA™4/TO/myc-His© Vector

Description

Includes

20μg of pcDNA4/TO or 20μg each of pcDNA4/TO/myc-His A, B, and C; a positive control vector, pcDNA6/TR, and forward and reverse sequencing primers. All vectors are supercoiled and provided lyophilized.

Tetracycline-regulated expression system without viral transactivators

• Uses complete CMV promoter and adds control elements from bacterial tetracycline resistance operon to effectively repress and derepress transcription from one of strongest mammalian promoter sequences known
• Specific Activation: uses repressor mechanism that blocks transcription from powerful CMV promoter in absence of tetracycline
• Because T-REx System elements do not use viral transactivators, can achieve high-level expression from complete CMV promoter without secondary, non-specific activation of host genes
• Two tetracycline operator sequences (TetO₂) have been inserted between TATA box of CMV promoter and transcriptional start site
• TetO₂ sequence itself has no effect on expression: When tetracycline repressor protein (TR) is present, it effectively binds TetO₂ sites and blocks transcription initiation
• Tetracycline added to culture medium binds to, and changes conformation of, TR protein causing TR protein to release TetO₂ sites and derepressing transcription from CMV promoter: The result is high-level expression of gene of interest

• Expression levels can be modulated based on tetracycline concentration and can be induced to levels that are achieved with constitutive CMV expression vectors
• Complete CMV enhancer-promoter sequence containing two copies of tetracycline operator TetO₂ sequence for high-level regulated expression
• Zeocinor hygromycin resistance gene for effective selection of stable mammalian cell lines
• Large multiple cloning site to simplify cloning
• In addition, pcDNA™4/TO/myc-His offers a c-myc epitope for rapid detection of recombinant protein with Anti-myc Antibody and polyhistidine (6xHis) sequence for simple purification of recombinant protein with nickel-chelating resin and detection with Anti- His(C-term) Antibody
• Regulatory vector, pcDNA™6/TR, is provided for high-level expression of tetracycline repressor (TR) protein
• Vector expresses Blasticidin resistance gene for rapid selection of mammalian cell lines that stably express TR protein

Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Regulated Expression

Specifications

• Constitutive or Inducible System: Inducible
• Delivery Type: Transfection
• Inducing Agent: Tetracycline
• Promoter: CMV/TO
• Product Type: T-Rex Core Cloning Kit with Vector
• Selection Agent (Eukaryotic): Zeocin™
• Content And Storage: The T-REx™ Core Kit includes 20 μg each of pcDNA™4/TO/myc-His A, B, and C; a positive control vector; pcDNA™6/TR; and forward and reverse sequencing primers.
  
  All vectors are supercoiled and provided lyophilized.
  
  Store all components at -20°C. All components are guaranteed stable for 6 months when properly stored.
• Protein Tag: His Tag (6x), c-Myc Epitope Tag
• Cloning Method: Restriction Enzyme/MCS
• Quantity: 1 kit
• Vector: pcDNA
• Product Line: T-REx™, pcDNA™
• For Use With (Application): Regulated Expression

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.