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Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ TOP10 Chemically Competent E. coli

Catalog No. K204001
Encompass_Preferred
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No. of Reactions:
20 Reactions
40 Reactions
Vector:
pCR2.1
pCRII

Provides a quick, one-step cloning strategy for directly inserting a Taq-amplified PCR product into a plasmid vector. Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ TOP10 Chemically Competent E. coli is available in 20- and 40-reaction kit sizes.

The TA Cloning™ Kit uses the pCR™2.1 cloning vector and ExpressLink™ T4 DNA Ligase to generate a ligation product in a fifteen-minute, room-temperature ligation step. Reactions typically yield >80% recombinants containing inserts.

  • Fast & Convenient—15-minute, room-temperature ligation
  • Efficient—Blue/white screening and >80% clones with correct insert
  • Flexible—Choice of kanamycin or ampicillin resistance for flexible antibiotic selection
  • Hassle-free—Eliminates any enzymatic modifications of the PCR product
  • Streamlined—Does not require the use of PCR primers that contain restriction sites

The pCR™2.1 vector provides:

  • 3′-T overhangs for direct ligation of Taq-amplified PCR products
  • T7 promoter for in vitro RNA transcription and sequencing
  • Versatile polylinker with flanking EcoR I sites for easy excision of inserts
  • M13 forward and reverse primer sites for sequencing

How TA Cloning™ Works
Taq polymerase has a non-template-dependent activity that adds a single deoxyadenosine (A) to the 3′ ends of PCR products. The linearized vector supplied in this kit has single 3′ deoxythymidine (T) residues. This allows PCR inserts to ligate efficiently with the vector.

Other Kit Configurations
TA Cloning™ Kits are also offered without competent cells (K2020-20, K2020-40), with One Shot™ INVF Chemically Competent E. coli (K2000-01, K2000-40) or with One Shot™ TOP10F Chemically Competent E. coli (K2030-01, K2030-40).

TRUSTED_SUSTAINABILITY

Specifications

Bacterial or Yeast Strain TOP10
Cell Type Chemically Competent E. coli
Cloning Method TA Cloning
Content And Storage TA Cloning™ kits contain linearized pCR™2.1 vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. The competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid.

Store One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Format Kit
For Use With (Application) PCR Cloning
No. of Reactions 20 Reactions
Product Line One Shot™
Product Type Cloning Kit
Promoter T7
Quantity 20 reactions
Vector pCRII
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For Research Use Only. Not for use in diagnostic procedures.

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