Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ TOP10 Chemically Competent E. coli

Description

Provides a quick, one-step cloning strategy for directly inserting a Taq-amplified PCR product into a plasmid vector. Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ TOP10 Chemically Competent E. coli is available in 20- and 40-reaction kit sizes.

The TA Cloning™ Kit uses the pCR™2.1 cloning vector and ExpressLink™ T4 DNA Ligase to generate a ligation product in a fifteen-minute, room-temperature ligation step. Reactions typically yield >80% recombinants containing inserts.

• Fast & Convenient—15-minute, room-temperature ligation
• Efficient—Blue/white screening and >80% clones with correct insert
• Flexible—Choice of kanamycin or ampicillin resistance for flexible antibiotic selection
• Hassle-free—Eliminates any enzymatic modifications of the PCR product
• Streamlined—Does not require the use of PCR primers that contain restriction sites

The pCR™2.1 vector provides:

• 3′-T overhangs for direct ligation of Taq-amplified PCR products
• T7 promoter for in vitro RNA transcription and sequencing
• Versatile polylinker with flanking EcoR I sites for easy excision of inserts
• M13 forward and reverse primer sites for sequencing

How TA Cloning™ Works
Taq polymerase has a non-template-dependent activity that adds a single deoxyadenosine (A) to the 3′ ends of PCR products. The linearized vector supplied in this kit has single 3′ deoxythymidine (T) residues. This allows PCR inserts to ligate efficiently with the vector.

Other Kit Configurations
TA Cloning™ Kits are also offered without competent cells (K2020-20, K2020-40), with One Shot™ INVF Chemically Competent E. coli (K2000-01, K2000-40) or with One Shot™ TOP10F Chemically Competent E. coli (K2030-01, K2030-40).

Specifications

• Bacterial or Yeast Strain: TOP10
• Cell Type: Chemically Competent E. coli
• Cloning Method: TA Cloning
• Content And Storage: TA Cloning™ kits contain linearized pCR™2.1 vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. The competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid.
  
  Store One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
• Format: Kit
• For Use With (Application): PCR Cloning
• No. of Reactions: 20 Reactions
• Product Line: One Shot™
• Product Type: Cloning Kit
• Promoter: T7
• Quantity: 20 reactions
• Vector: pCRII

For Research Use Only. Not for use in diagnostic procedures.