Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ TOP10F' Chemically Competent E. coli

Description

Provides quick, one-step cloning strategy for directly inserting a Taq-amplified PCR product into plasmid vector

The TA Cloning Kit uses the pCR2.1 cloning vector and ExpressLink™ T4 DNA Ligase to generate a ligation product in a fifteen-minute, room-temperature ligation step. Reactions typically yield >80% recombinants containing inserts.

• Fast and convenient: 15-minute, room-temperature ligation
• Efficient: blue/white screening and >80% clones with correct insert
• Flexible: choice of kanamycin or ampicillin resistance for flexible antibiotic selection
• Hassle-free: eliminates any enzymatic modifications of PCR product
• Streamlined: does not require use of PCR primers that contain restriction sites
• 3'-T overhangs for direct ligation of Taq-amplified PCR products
• T7 promoter for in vitro RNA transcription and sequencing
• Versatile polylinker with flanking EcoR I sites for easy excision of inserts
• M13 forward and reverse primer sites for sequencing

• How TA Cloning™ Works
• Taq polymerase has non-template-dependent activity that adds single deoxyadenosine (A) to 3' ends of PCR products
• Llinearized vector supplied in this kit has single 3' deoxythymidine (T) residues
• Allows PCR inserts to ligate efficiently with vector
• Kit Configurations
• TA Cloning™ Kit is offered in a variety of configurations: without competent cells (K2020-20 and K2020-40), with One Shot™ INVF' Chemically Competent E. coli (K2000-01 and K2000-40), with One Shot™ TOP10F' Chemically Competent E. coli (K2030-01 and K2030-40), and with One Shot™ TOP10 Chemically Competent E. coli (K2040-01 and K2040-40) in 20- and 40-reaction kit sizes

Cloning, PCR Cloning

Specifications

• Bacterial or Yeast Strain: TOP10F ́
• Cell Type: Chemically Competent E. coli
• Cloning Method: TA Cloning
• Content And Storage: TA Cloning™ kits contain linearized pCR™2.1 vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. The competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid.
  
  Store One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
• Format: Kit
• For Use With (Application): PCR Cloning
• No. of Reactions: 40 Reactions
• Product Line: One Shot™
• Product Type: Cloning Kit
• Promoter: T7
• Quantity: 40 reactions
• Vector: pCR2.1

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.