Invitrogen™ TE Buffer

Catalog No.	Quantity
12-090-015	100 mL

Catalog No. 12-090-015 Supplier Invitrogen™ Supplier No. 12090015

Description

This 1X TE Buffer is a component of the PureLink™ 96 Plasmid Purification System, now offered separately.

This 1X TE Buffer is a component of the PureLink™ 96 Plasmid Purification System, now offered separately. It is used to resuspend the final purified plasmid pellet and contains very low EDTA, so it is compatible with sequencing and other enzymatic applications.

Composition:
10 mM Tris-HCl (pH 8.0)
0.1 mM EDTA

Order Info

Shipping Condition: Room Temperature

Specifications

• Concentration: 1 X
• Content And Storage: Store at room temperature.
• Product Type: TE Buffer
• Quantity: 100 mL
• Shipping Condition: Room Temperature
• Form: Liquid

Frequently Asked Questions (FAQs)

How do I download the Pathogen Interpretive Software?

Once you place your order, please contact our Instrument Service team at 1-800-955-6288 option 3, 1 (this kit is only available in North America). You will be given an access code to the TaqPath Monkeypox/Orthopox Virus DNA Kit eLearning where you’ll learn how to install, update, and download the Pathogen Interpretive Software package for the TaqPath Monkeypox/Orthopox Virus DNA Kit.

With the TaqPath Monkeypox/Orthopox Virus DNA Kit, what software should I use for data analysis?

Results are analyzed using the following software:
- Applied Biosystems Pathogen Interpretive Software v1.1
- SAE Administrator Console Dx v1.0 or v1.2 (for security and audit functions)
- Pathogen Interpretive Software 1.0.0 DAT (SAE Profile)

Please use the appropriate assay panel and template for your instrument:
- MPXOPX-EUA_QS5-9601_1.0.0.zip
- MPXOPX-EUA_QS5Dx-9602_1.0.0.zip
- MPXOPX-EUA_QS7F-384_1.0.0.zip

Note: These zip files are located in EducationConnect (www.thermofisher.com/educationconnect). The eLearning course needs to be completed to be granted access.

What is the instrument software configuration required to use the TaqPath Monkeypox/Orthopox Virus DNA Kit?

To obtain the correct analysis software or firmware version for your real-time PCR instrument, go to thermofisher.com/validatedfirmware, then select your instrument in the Real-Time PCR section.

Which instruments are authorized under the EUA for use with the TaqPath Monkeypox/Orthopox Virus DNA Kit?

Here are the instruments that can be used with the TaqPath Monkeypox/Orthopox Virus DNA Kit:
- Applied Biosystems QuantStudio 5 Real-Time PCR System (96-well, 0.1 mL block)
- Applied Biosystems QuantStudio 5 Dx Real-Time PCR System (96-well, 0.2 mL block)
- Applied Biosystems QuantStudio 7 Flex Real-Time PCR System (384-well block)

I would like to use the TaqPath Monkeypox/Orthopox Virus DNA Kit. Which extraction kit should I use to extract DNA from the sample?

Performance was established using the MagMAX Viral/Pathogen II Nucleic Acid Isolation Kit (Cat. No. A48383) in combination with the KingFisher Flex Magnetic Particle Processor with 96 Deep-Well Head and the KingFisher Flex 96 Deep-Well Heating Block (MVP_2Wash_400_Flex program). Other purification methods may lead to deviations in the expected result.

Which specimen types are compatible with the TaqPath Monkeypox/Orthopox Virus DNA Kit?

The TaqPath Monkeypox/Orthopox Virus DNA Kit protocol is currently authorized for use with lesion swab specimens in universal transport media (UTM) or viral transport media (VTM) from individuals suspected of monkeypox infection by their healthcare provider.

What instruments, consumables, and reagents do I need for the TaqPath Monkeypox/Orthopox Virus DNA Kit? Where can I find the full list of EUA products and protocols?

Go to www.thermofisher.com/monkeypox and download the Instructions for Use (IFU) to see the workflow outlining the instruments, consumables, and reagents required to run Monkeypox detection experiments.

What are the reporter dyes and targets for the TaqPath Monkeypox/Orthopox Virus DNA Kit?

The reporter dyes used are as follows:
- Monkeypox virus: FAM dye
- Orthopoxvirus: VIC dye
- RNase P: JUN dye

The TaqPath Monkeypox/Orthopox Virus DNA Kit probes contain QSY and MGB quenchers, which do not fluoresce. Select None for Quencher in the instrument set up procedure.

What are the components of the TaqPath Monkeypox/Orthopox Virus DNA Kit? Is the master mix included, or does it need to be purchased separately? Are there any other required materials that are not supplied?

The TaqPath Monkeypox/Orthopox Virus DNA Kit includes the following components:
- TaqPath Monkeypox/Orthopox Virus Multiplex Assay: Multiplexed real-time PCR assay containing primers/probes specific to monkeypox virus, non-variola orthopoxvirus targets, and human RNase P.
- TaqPath Monkeypox/Orthopox Virus DNA Positive Control: Positive DNA control that contains templates specific to monkeypox virus, non-variola orthopoxvirus targets, and human RNase P regions targeted by the assay.

Note:
- The master mix is not included in the kit and needs to be purchased separately: TaqPath BactoPure Microbial Detection Master Mix (No ROX) (Cat. No. A52703, A52704, or A52705)
- TE Buffer (Cat. No. 12090015) and Nuclease-free water (MLS) are required and not supplied with the kit.

For Research Use Only. Not for use in diagnostic procedures.