Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with One Shot™ MAX Efficiency™ DH5α-T1^R E. coli

Catalog No.	Quantity
K462040	50 Reactions
K462001	25 Reactions

Catalog No. K462040 Supplier Invitrogen™ Supplier No. K462040

Description

Includes

Topoisomerase I-activated pCR™II-TOPO™ vector; PCR buffer; Salt solution; dNTPs; Control template; T3, T7, M13 forward, and M13 reverse primers; Control PCR primers; Sterile water; One Shot™ Chemically Competent or Electrocomp™ E. coli; S.O.C. medium; Supercoiled pUC19 control plasmid

Designed for cloning PCR products directly from a PCR reaction in just 5 minutes

Uses a pCR™-TOPO™ Vector with covalently bound topoisomerase I for fast cloning and recombinants.

• 3'-T overhangs for direct ligation of Taq-amplified PCR products
• Choice of T7 (pCR2.1-TOPO) or T7 and SP6 (pCRII-TOPO) promoters for in vitro RNA transcription and sequencing. Each vector also contains M13 forward and reverse primer sites for sequencing.
• EcoR I sites flanking the PCR product insertion site for easy excision of inserts
• Kanamycin and ampicillin resistance genes for your choice of selection in E. coli
• Easy blue/white colony screening for selection of recombinants

Cloning, PCR Cloning

Specifications

• Bacterial or Yeast Strain: DH5α
• Cloning Method: TOPO™-TA
• Content And Storage: Box 1:
  • Topoisomerase I-activated pCR™II-TOPO™ vector
  • PCR buffer
  • Salt solution
  • dNTPs
  • Control template
  • M13 forward and reverse primers
  • Control PCR primers
  • Sterile water
  
  Store at -5 to -30°C.
  All reagents are stable for 6 months when properly stored.
  
  Box 2:
  • One Shot™ Chemically Competent or Electrocomp™ E. coli
  • S.O.C. medium
  • Supercoiled pUC19 control plasmid.
  
  Store at -68 to -85°C.
• Format: Kit
• Product Line: One Shot
• Product Type: Cloning Kit
• Promoter: T7, SP6
• Quantity: 50 Reactions
• Vector: TOPO-TA Cloning Vectors

For Research Use Only. Not for use in diagnostic procedures.