Invitrogen™ TOPO™ TA Cloning™ Kit, with pCR™2.1-TOPO™, One Shot Mach1™-T1R Chemically Competent E. coli, and PureLink™ Quick Plasmid Miniprep Kit

Designed for cloning PCR products directly from a PCR reaction in just 5 minutes

Supplier:  Invitrogen™ K451022

Catalog No. K451022

Includes: Topoisomerase I-activated pCR™2.1-TOPO™ vector; PCR buffer; Salt solution; dNTPs; Control template; M13 forward and reverse primers; Control PCR primers; Sterile water; One Shot™ Chemically Competent or Electrocomp™ E. coli; S.O.C. medium; Supercoiled pUC19 control plasmid

Description

Uses a pCR™-TOPO Vector with covalently bound topoisomerase I for fast cloning and recombinants.

• 3'-T overhangs for direct ligation of Taq-amplified PCR products
• Choice of T7 (pCR2.1-TOPO™) or T7 and SP6 (pCRII-TOPO™) promoters for in vitro RNA transcription and sequencing. Each vector also contains M13 forward and reverse primer sites for sequencing.
• EcoR I sites flanking the PCR product insertion site for easy excision of inserts
• Kanamycin and ampicillin resistance genes for your choice of selection in E. coli
• Easy blue/white colony screening for selection of recombinants
• TOPO TA Cloning Kits are available in combo kits combined with a PureLink™ Quick Plasmid Miniprep Kit (50 preps) for fast plasmid purification of your TOPO-cloned inserts for downstream analysis

Cloning, PCR Cloning

Specifications

• Bacterial or Yeast Strain: Mach1™
• Cloning Method: TOPO™-TA
• Format: Kit
• Product Type: Cloning Kit
• Quantity: 25 Reactions

• Cell Type: Chemically Competent
• Content And Storage: Box 1:
  • Topoisomerase I-activated pCR™2.1-TOPO™ vector
  • PCR buffer
  • Salt solution
  • dNTPs
  • Control template
  • M13 forward and reverse primers
  • Control PCR primers
  • Sterile water
  
  Store at -5 to -30°C.
  All reagents are stable for 6 months when properly stored.
  
  Box 2:
  • One Shot™ Chemically Competent or Electrocomp™ E. coli
  • S.O.C. medium
  • Supercoiled pUC19 control plasmid
  
  Store at -68 to -85°C.
• Product Line: One Shot™
• Promoter: T7, SP6
• Vector: TOPO-TA Cloning Vectors

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.