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Invitrogen™ TOPO™ TA Cloning™ Kit, with pCR™2.1-TOPO™, One Shot Mach1™-T1R Chemically Competent E. coli, and PureLink™ Quick Plasmid Miniprep Kit

Designed for cloning PCR products directly from a PCR reaction in just 5 minutes

Manufacturer:  Invitrogen™ K451022

Encompass

Catalog No. K451022


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Includes: Topoisomerase I-activated pCR™2.1-TOPO™ vector; PCR buffer; Salt solution; dNTPs; Control template; M13 forward and reverse primers; Control PCR primers; Sterile water; One Shot™ Chemically Competent or Electrocomp™ E. coli; S.O.C. medium; Supercoiled pUC19 control plasmid

Description

Description

Uses a pCR™-TOPO Vector with covalently bound topoisomerase I for fast cloning and recombinants.

  • 3'-T overhangs for direct ligation of Taq-amplified PCR products
  • Choice of T7 (pCR2.1-TOPO™) or T7 and SP6 (pCRII-TOPO™) promoters for in vitro RNA transcription and sequencing. Each vector also contains M13 forward and reverse primer sites for sequencing.
  • EcoR I sites flanking the PCR product insertion site for easy excision of inserts
  • Kanamycin and ampicillin resistance genes for your choice of selection in E. coli
  • Easy blue/white colony screening for selection of recombinants
  • TOPO TA Cloning Kits are available in combo kits combined with a PureLink™ Quick Plasmid Miniprep Kit (50 preps) for fast plasmid purification of your TOPO-cloned inserts for downstream analysis

Cloning, PCR Cloning

Specifications

Specifications

Cloning Kit
Mach1™
TOPO-TA Cloning Vectors
Kit
25 Reactions
Chemically Competent
TOPO™-TA
T7, SP6
One Shot™
Safety and Handling

Safety and Handling

SDS
Documents
Product Certifications
Promotions

Promotions

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For Research Use Only. Not for use in diagnostic procedures.