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Invitrogen™ UltraPure™ Dithiothreitol

Catalog No. 15508013
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Catalog No. 15-508-013 Supplier Invitrogen™ Supplier No. 15508013
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Description

UltraPure Dithiothreitol (DTT, Cleland's reagent), which reduces disulfides to their corresponding thiols, is used at low concentrations to stabilize enzymes containing free sulfhydryl groups.

UltraPure Dithiothreitol (DTT, Cleland's reagent), which reduces disulfides to their corresponding thiols, is used at low concentrations to stabilize enzymes containing free sulfhydryl groups. Higher concentrations of DTT are used to cleave disulfide linkages in polypeptides and to facilitate protein denaturation by detergents or chaotropic agents.

Order Info

Approved for shipment at Room Temperature or on Wet Ice

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Store in freezer (-5° to -30°C).
For Use With (Application) Protein Electrophoresis & Western Blotting
Product Line UltraPure
Quantity 5 g
Shipping Condition Approved for shipment at Room Temperature or on Wet Ice

Frequently Asked Questions (FAQs)

Can beta-mercaptoethanol (BME) be used rather than DTT as the reducing agent in the NuPAGE LDS Sample Buffer?

Either BME or DTT can be used in the NuPAGE LDS Sample Buffer.

Make sure that a fresh solution of BME is used. FINAL concentration:

DTT 50-100 mM

BME 2-5%
How do I prepare a 1 M DTT solution from powder and how should I store the solution?

Preparing 1 M DTT:
1) Dissolve 3.09 g DTT in 20 mL distilled H2O.
2) Dispense into 1 mL aliquots.
3) Store the aliquots at -20 degrees C.
Note: Upon thawing the vial, there may be some precipitation that can be re-dissolved by warming and mixing.

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

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