Invitrogen™ UltraPure™ TBE Buffer, 10X

Catalog No.	Quantity
15-581-028	10 L
15-581-044	1 L

Catalog No. 15-581-028 Supplier Invitrogen™ Supplier No. 15581028

Description

Compatible with agarose gels

UltraPure 10X TBE Buffer is a sterile-filtered solution of 1M Tris, 0.9 M boric acid, and 0.01M EDTA used to prepare 1X buffer for polyacrylamide and agarose gel electrophoresis.

• UltraPure 10X TBE Buffer is available in 1 L plastic bottle/10 L Cubitainer™ box
• Performance and quality testing: No DNase, RNase, or protease activity detected. Sustainable packaging

Acrylamide Gel Electrophoresis, DNA & RNA Purification & Analysis, Nucleic Acid Gel Electrophoresis & Blotting

Order Info

Shipping Condition: Room Temperature

Specifications

• Buffer: Running Buffer
• Concentration: 10 X
• Content And Storage: Store at room temperature.
• Format: Bottle
• Gel Compatibility: Polyacrylamide Gels, Agarose Gels
• For Use With (Application): Nucleic Acid Gel Electrophoresis, Blotting
• Product Line: UltraPure
• Product Type: TBE Buffer
• Quantity: 10 L
• Shipping Condition: Room Temperature

Frequently Asked Questions (FAQs)

Can I autoclave agarose to prepare agarose gels?

No. Autoclaving at temperatures that are too high or pressures that are too high can hydrolyze the agarose polymer and weaken it.

Do you have any tips for preparing high-percentage (3% to 4%) agarose gels?

Add the powder to cold buffer while stirring. Let the agarose rehydrate for 1 to 2 hr and then heat slowly until agarose is completely dissolved. Using a microwave at low power settings is acceptable.

What can I do to prevent static on agarose bottles?

One recommendation: get a supply of fabric softener sheets and wipe the bottle with a sheet before opening it.

How can I avoid the build-up of the precipitous film sometimes seen in 10X TBE?

The precipitation of concentrated TBE stocks may be due to nucleation of salt crystals by dust particles or other insoluble materials. Therefore, filtering the solution through a 0.2 µm cellulose acetate or cellulose nitrate filter after preparation helps avoid this precipitate. [Mayeda A, Krainer A (1991) BioTechniques 10.2, 1820]

How can generation of replication competent adenoviruses be avoided when using your pSilencer adeno 1.0-CMV System?

Although there is only a very small chance of creating replication competent virus, steps should still be taken to avoid added risk. The virus is expanded in two rounds of amplification, and all secondary expansions should be performed from an initial expansion stock. Secondary amplifications in HEK293 cells should not be performed from the stock of another secondary expansion, as this could increase the chance of making replication competent virus. See the product manual for more detail and guidelines for screening.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.