Invitrogen™ ViraPower™ Lentiviral Directional TOPO™ Expression Kit

Description

Includes

pLenti6/V5 Directional TOPO Cloning Kit: pLenti6/V5 Directional TOPO Reagents, Stbl3 Chemically Competent E. coli, ViraPower Bsd Lentiviral Support Kit: ViraPower Packaging Mix, Lipofectamine 2000, Blasticidin, 293FT Cell Line

Facilitates quick PCR-based cloning and lentiviral-based expression of target gene in dividing and nondividing mammalian cells

• Includes all components needed to generate lentivirus, including vector kit, 293FT cell line, and support kit
• PLenti6/V5-D-TOPO vector has the CMV promoter for driving constitutive expression of the target gene and blasticidin selection marker for stable selection in mammalian cells
• Constitutive high expression with CMV promoter
• Blasticidin selection marker for stable selection
• C terminal V5 tag for quick detection

Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Viral Gene Delivery

Specifications

• Constitutive or Inducible System: Constitutive
• Delivery Type: Lentiviral
• Promoter: CMV
• Product Type: Lentiviral Expression Kit
• Selection Agent (Eukaryotic): Blasticidin
• Content And Storage: pLenti6⁄V5™ Directional TOPO™ Cloning Kit:
  • pLenti6⁄V5™ Directional TOPO™ Reagents: -20°C
  • One Shot™ Stbl3™ Chemically Competent E. coli:-80°C
  
  ViraPower™ Bsd Lentiviral Support Kit:
  • ViraPower™ Packaging Mix: -20°C
  • Lipofectamine™ 2000: 4°C (DO NOT freeze)
  • Blasticidin: -20°C
  • 293FT Cell Line: liquid nitrogen
  
• Protein Tag: V5 Epitope Tag
• Cloning Method: Directional TOPO
• Quantity: 1 kit
• Vector: Directional TOPO Vectors
• Product Line: One Shot™
• For Use With (Application): Viral Expression

For Research Use Only. Not for use in diagnostic procedures.