Invitrogen™ Yeast Nitrogen Base

Catalog No.	Quantity
Q30007	1 Pouch

Catalog No. Q30007 Supplier Invitrogen™ Supplier No. Q30007

Description

Includes

Each pouch contains reagents to prepare 500mL of 10X YNB for Pichiamedia or 1000mL of 10X YNB for S. cerevisiaemedia.

Base medium for preparation of minimal and synthetic defined yeast media

• Contains ammonium sulfate but does not contain amino acids
• Powder

Cloning, Transformation

Specifications

• Content And Storage: Each pouch contains 67 g of reagent to prepare 500 mL of 10X YNB for Pichia media or 1,000 mL of 10X YNB for S. cerevisiae media.
  
  Store at room temperature. Guaranteed stable for 6 months when properly stored.
• Form: Powder
• Product Type: Microbiology Media
• Target Organism Class: P. pastoris, S. cerevisiae
• Quantity: 1 Pouch

Frequently Asked Questions (FAQs)

What are the components and concentrations for 1X YNB (yeast nitrogen base)?

For the pouches sold as Cat. No. Q30007, each pouch contains reagents to prepare 500 mL of 10X YNB for Pichia media or 1,000 mL of 10X YNB for S. cerevisiae media (since Pichia media is 2X what is used for S. cerevisiae). Below is the formulation for 1X YNB:

Ammonium sulfate, 5 g
Biotin, 0.002 mg
Calcium pantothenate, 0.4 mg
Folic acid, 0.002 mg
Inositol, 2 mg
Niacin, 0.4 mg
Para-aminobenzoic acid, 0.2 mg
Pyridoxine HCl, 0.4 mg
Riboflavin, 0.2 mg
Thiamine HCl, 0.4 mg
Boric acid, 0.5 mg
Copper sulfate, 0.4 mg
Potassium iodide, 0.1 mg
Ferric chloride.6H2O, 0.2 mg
Manganese sulfate.H2O, 0.4 mg
Sodium molybdate.2H2O, 0.2 mg
Zinc sulfate.H2O, 0.4 mg
Potassium phosphate monobasic, 1 g
Magnesium sulfate, 0.5 g
Sodium chloride, 0.1 g
Calcium chloride, 0.1 mg

Can YNB medium (with ammonium sulfate, without amino acids) be autoclaved instead of filter-sterilized?

Yes, YNB medium with ammonium sulfate and without amino acids can be autoclaved for sterilization.

For Research Use Only. Not for use in diagnostic procedures.