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Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ TOP10 Electrocomp™ E. coli
Description
Includes
Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector; Salt solution; dNTPs; Control template; M13 forward and reverse primers; Sterile water; One Shot™ Chemically Competent or Electrocomp™ E. coli; S.O.C. medium; Supercoiled pUC19 control plasmid
Kit includes linearized and topoisomerase I-activated pCR™-Blunt II-TOPO Vector for 5-minute benchtop ligations without ligase.
- ccdB gene for positive selection
- EcoR I sites flanking the PCR product insertion site for easy excision of inserts
- Kanamycin and Zeocin™ resistance genes for your choice of selection in E. coli
- SP6 promoter/primer site for in vitro RNA transcription and sequencing
- M13 forward and reverse primer sites for sequencing or PCR screening
- Selected Zero Blunt TOPO PCR Cloning Kits are available in combo kits combined with a PureLink™ Quick Plasmid Miniprep Kit (50 preps) for fast plasmid purification of your TOPO-cloned inserts for downstream analysis
Cloning, PCR Cloning
Specifications
Specifications
| Bacterial or Yeast Strain | TOP10 |
| Cloning Method | Blunt TOPO™ |
| Content And Storage | Box 1: • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid Store at -68 to -85°C. |
| Format | Kit |
| Product Line | One Shot™ |
| Product Type | PCR Cloning Kit |
| Promoter | SP6 |
| Quantity | 50 Reactions |
| Vector | Blunt DNA Cloning Vectors |
Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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