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Invitrogen™ Zero Blunt™ TOPO PCR Cloning Kit for Subcloning
Description
Includes
Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector; Salt solution; dNTPs; Control template; M13 forward and reverse primers; Sterile water; One Shot™ Chemically Competent or Electrocomp™ E. coli; S.O.C. medium; Supercoiled pUC19 control plasmid
- Unique technology to minimize background
- Get your clone: 95% efficiency
- Multiple primer sites (T7, T3, M13F, M13R) make sequence analysis or PCR easy and convenient
- Kanamycin and Zeocin resistance genes for your choice of selection
- Convenient, validated restriction sites in MCS for further subcloning
- Includes MachI™ T1R competent cells for fast miniprep and shortens cloning time
Chromatin Biology, Chromatin Immunoprecipitation (ChIP), Cloning, Methylation Analysis, PCR Cloning, RNAi, Epigenetics and Non-Coding RNA Research, Sequencing Bisulfite-Treated DNA
Specifications
Specifications
| Bacterial or Yeast Strain | Mach1™ |
| Cloning Method | Blunt TOPO™ |
| Content And Storage | Box 1: • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid Store at -68 to -85°C. |
| Format | Kit |
| For Use With (Application) | Chromatin Biology |
| Product Line | One Shot™ |
| Product Type | PCR Cloning Kit |
| Quantity | 25 Reactions |
| Vector | Blunt DNA Cloning Vectors |
For Research Use Only. Not for use in diagnostic procedures.
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