ReproSeq™ PGS Kit with Ion 530™ Chips (96 samples/run)

Catalog No.	Quantity
A34901	384 samples

Catalog No. A34901 Supplier LIFE TECHNOLOGIES Supplier No. A34901

Description

The ReproSeq PGS Kit for the Ion S5 System provides all the reagents and materials necessary for a rapid, cost-effective, and comprehensive next-generation sequencing (NGS) workflow for aneuploidy analysis.

When used with an Ion S5 sequencing system and Ion Chef System for automated template preparation, the kit enables a simple, end-to-end workflow for the detection of aneuploidies across all 24 chromosomes (22 autosomes and the X and Y chromosomes) for research labs interested in reliably assessing pre-implantation embryo biopsy samples.

Using as little as 6 pg of DNA from a single cell, or input from multiple cells, the ReproSeq PGS Kit for the Ion S5 System enables accurate detection of whole chromosome and chromosome arm copy number events (down to 17 Mbp copy number events*) in as little as 10 hours**. Three kit configurations provide comprehensive sample throughput flexibility enabling cost-effective screening of 16, 24, or 96 samples per run. When combined with analysis using Ion Reporter v5.4 or later, data interpretation is enhanced with new functionality such as mosaicism detection, gender masking, and improved data plotting.

Cost-effective NGS workflow with sample throughput flexibility
The ReproSeq PGS Kit for the Ion S5 System delivers scalability and flexibility through three sequencing chips—the Ion 510 Chip, Ion 520 Chip, and Ion 530 Chip—to support various levels of sample throughput. Choose the kit that fits your throughput needs to process 16, 24, and up to 96 samples to achieve throughput flexibility that scales up to improve cost efficiencies and allows your laboratory to respond to sample fluctuation and increases in sample volume.

Rapid turnaround for time-sensitive samples
Leveraging the speed of semiconductor sequencing and automated template preparation by isothermal amplification on the Ion Chef System, the ReproSeq PGS Kit for the Ion S5 System offers a fast benchtop NGS workflow for aneuploidy analysis studies. Go from a single cell or multiple cells isolated from a pre-implantation embryo to analyzed data typically in less than 10 hours when processing up to 16 samples, 10 hours for 24 samples, or 13 hours for up to 96 samples. This rapid turnaround time enables reliable identification of chromosomally normal embryos for time-sensitive genetic research.

The ReproSeq PGS Kit with Ion 530 Chips (96 samples/run) includes all reagents for processing a total of 384 samples through a workflow that includes whole genome amplification, library construction, template preparation and chip loading on the Ion Chef instrument, and sequencing using an Ion S5 System.

The kit contains:
• Ion SingleSeq 96 Kit (Cat. No. A34763)—includes reagents to extract, amplify, and barcode genomic DNA for preparing libraries from pre-implantation embryo biopsy samples, a process that can be completed in less than 5 hours (includes hands-on time)
• Ion S5 ExT Chef Supplies, Ion S5 Chef Solutions, and Ion S5 ExT Chef PGS Reagents—contains pre-packaged single-use cartridges for automated template preparation and chip loading (5 hours, includes hands-on time) whereby library DNA is clonally amplified onto a bead surface through an emulsion-based isothermal amplification reaction
• Ion S5 ExT Sequencing Solutions, Ion S5 ExT Sequencing Reagents—simplifies sequencing with ready-to-use, disposable reagent cartridges for fast and simple instrument set-up, with less than 15 minutes hands-on time, for a sequencing chemistry supporting advanced data quality and accuracy
• Ion 530 Chip Kit (Cat. No. A27763)—contains 4 barcoded chips per kit for sample tracking and sequencing runs using the Ion S5 System

Notes
• This kit is only compatible with the Ion Chef System and Ion S5 systems. The Ion S5 XL System is recommended for rapid turnaround time between sequencing runs. Use of the kits with the Ion S5 System requires significantly more analysis time between runs.
• The ReproSeq PGS Kit is compatible with Torrent Suite Software 5.4 and later. Update your Torrent Server to the latest available version of Torrent Suite Software before using these kits. For data analysis, Ion Reporter Software 5.4 or later is recommended.

*Detecting copy number events <48 Mbp using the ReproSeq™ PGS Kit with Ion 530™ Chips (96 samples/run) requires running ≤24 samples on an Ion 530 Chip
**Sample to analyzed results turnaround time for the ReproSeq PGS Kit with Ion 510 Chips (16 samples/run) is 9.6 hours.

Specifications

• Content And Storage: • Ion SingleSeq 96 Kit, 4 boxes, –10 to –30°C
  • Ion S5 ExT Chef Supplies, 4 boxes, 15–30°C
  • Ion S5 Chef Solutions, 4 cartridges, 15–30°C
  • Ion S5 ExT Chef PGS Reagents, 4 cartridges, –10 to –30°C
  • Ion S5 ExT Sequencing Solutions, 1 box, 15–30°C
  • Ion S5 ExT Sequencing Reagents, 4 cartridges, –10 to –30°C
  • Ion 530 Chip Kit, 4 chips, 15–30°C
  
• Format: Kit
• For Use With (Equipment): Ion GeneStudio S5 System, Ion PGM™ System, Ion Chef™ System
• For Use With (Application): Shotgun Sequencing, Rapid Sequencing
• No. of Samples: 384
• Product Line: Ion 530, ReproSeq
• Product Type: PGS Kit
• Quantity: 384 samples
• Shipping Condition: Approved for shipment on Wet or Dry Ice

Frequently Asked Questions (FAQs)

How many total samples can I process with the Ion ReproSeq PGS Kit with Ion 530 Chips?

384 samples in total (4 runs with 96 samples/run) can be processed with the Ion ReproSeq PGS Kit with Ion 530 Chips.

How many samples per run can I process with the Ion ReproSeq PGS Kit with Ion 530 Chips?

96 samples per run can be processed with the Ion ReproSeq PGS Kit with Ion 530 Chips.

What does the Ion ReproSeq PGS Kit with Ion 530 Chips (96 samples/run) include, and what are the storage conditions for each component?

*Ion SingleSeq 96 Kit, 4 boxes, -10 to -30 degrees C
*Ion S5 ExT Chef Supplies, 4 boxes, 15-30 degrees C
*Ion S5 Chef Solutions, 4 cartridges, 15-30 degrees C
*Ion S5 ExT Chef PGS Reagents, 4 cartridges, -10 to -30 degrees C
*Ion S5 ExT Sequencing Solutions, 1 box, 15-30 degrees C
*Ion S5 ExT Sequencing Reagents, 4 cartridges, -10 to -30 degrees C
*Ion 530 Chip Kit, 4 chips, 15-30 degrees C

Does the Ion SingleSeq 96 Kit include reagents for template preparation, sequencing, and chips?

No, the Ion SingleSeq 96 Kit only includes reagents to prepare the library. For a detailed list, please go here (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0016712_IonReproSeqPGS_S5_UG.pdf).

What is included in the Ion SingleSeq 96 Kit?

The Ion SingleSeq 96 Kit includes reagents to extract, amplify, and barcode genomic DNA for preparation of libraries from up to 96 samples. For a detailed list, please go here (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0016712_IonReproSeqPGS_S5_UG.pdf).

Where can I find the sequences for the IonCode barcodes?

The sequences for the barcodes can be found in the Barcodes section of your Torrent Browser. You can also download a .csv file containing the full list of sequences in that set.

Are the Ion ReproSeq PGS Kit subkits available separately?

Yes, the Ion SingleSeq Kit (Cat. No. A28955, 24 reactions/kit), or Ion SingleSeq 96 Kit (Cat. No. A34763, 96 reactions/kit) are available separately.

The Ion ReproSeq PGS Kits - Ion S5 System, are also available separately and include reagents to prepare templates and load 4 chips on the Ion Chef Instrument, and sequence the chips on the Ion S5 or Ion S5 XL Sequencer.
- Ion ReproSeq PGS Kit with Ion 520 Chips (24 samples/run), Cat. No. A34900
- Ion ReproSeq PGS Kit with Ion 530 Chips (96 samples/run), Cat. No. A34901

For Research Use Only. Not for use in diagnostic procedures.