Invitrogen™ Human IP-10/CXCL10 Instant ELISA™ Kit

Catalog No.	Quantity
50-182-34	128 Tests

Catalog No. 50-182-34 Supplier Invitrogen™ Supplier No. BMS284INST

Description

ELISA

The Human Inflammatory Protein-10 (Hu IP-10/CXCL10) ELISA quantitates Hu sIP-10/CXCL10 in human serum, plasma, cell culture supernatants, urine, amniotic fluid, synovial fluid or other body fluids. The assay will exclusively recognize both natural and recombinant Hu sIP-10/CXCL10. Principle of the method The Human sIP-10/CXCL10 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IP-10 (interferon-gamma induced protein 10 kD; in mouse, also called cytokine responsive gene 2, Crg-2, or mob-1) is a highly inducible, primary response gene that belongs to the CXC chemokine superfamily. It was first cloned in 1985. The biological functions of IP-10 are still unclear. Like Mig, IP-10 has no activity on neutrophils. Its functions include stimulation of monocytes, natural killer and T-cell migration, regulation of T-cell and bone marrow progenitor maturation, modulation of adhesion molecule expression as well as inhibition of angiogenesis. Mig and IP-10 share the same receptor, CXCR3.

Specifications

• Accession Number: P02778
• Assay Range: 3.1-200 pg/mL
• Assay Sensitivity: 1.0 pg/mL
• Content And Storage: Aluminum pouch(es) with a Microplate coated with Monoclonal Antibody (murine) to human IP-10, Biotin-Conjugate (anti-IP-10 monoclonal antibody), Sample Diluent, and Streptavidin-HRP, lyophilized
  Aluminum pouch(es) with a human IP-10 Standard curve (coloured)
  Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)
  Substrate Solution (tetramethyl-benzidine)
  Sample Diluent (Use when an external predilution of the samples is needed)
  Stop Solution (1M Phosphoric acid)
  Adhesive Plate Covers
• Conjugate: HRP
• Immunoassay Kit Format: Coated ELISA Kit
• Product Type: ELISA
• Sample Type: Amniotic Fluid, Plasma, Serum, Supernatant, Synovial Fluid, Urine
• Target Species: Human
• For Use With (Application): ELISA
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 10 kDa interferon gamma-induced protein; 10 kDa interferon-gamma induced protein precursor; C Cmotif chemokine; C X C motif chemokine; C7; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 10; chemokine C-X-C motif ligand 10; chemokine CXC-like protein; Crg2; CRG-2; CXC; CXC chemokine; CXC motif chemokine; C-X-C motif chemokine 10; C-X-C motif chemokine 10-like protein; C-X-C motif chemokine ligand 10; CXCL; CXCL10; CXCL10(1-73); gamma interferon inducible protein 10; gamma IP10; gamma-IP10; gIP-10; H-IP-10; IFI10; Inp10; interferon activated gene 10; interferon-gamma induced protein CRG-2; interferon-gamma-induced protein CRG-2; interferon-gamma-inducible protein 10; interferon-gamma-inducible protein-10; interferon-inducible cytokine IP-10; interferon-inducible protein 10; IP10; IP-10; M-IP-10; Mob1; mob-1; protein 10 from interferon (gamma)-induced cell line; protein Mob-1; Scyb10; similar to Small inducible cytokine B10 precursor (CXCL10) (10 kDa interferon-gamma induced protein) (Gamma-IP10) (IP-10); small inducible cytokine B subfamily (Cys-X-Cys) member 10; small inducible cytokine B subfamily (Cys-X-Cys), member 10; small inducible cytokine subfamily B (Cys-X-Cys), member 10; small-inducible cytokine B1010 kDa interferon gamma-induced protein; 10 kDa interferon-gamma induced protein precursor; C Cmotif chemokine; C X C motif chemokine; C7; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 10; chemokine C-X-C motif ligand 10; chemokine CXC-like protein; Crg2; CRG-2; CXC; CXC chemokine; CXC motif chemokine; C-X-C motif chemokine 10; C-X-C motif chemokine 10-like protein; C-X-C motif chemokine ligand 10; CXCL; CXCL10; CXCL10(1-73); gamma interferon inducible protein 10; gamma IP10; gamma-IP10; gIP-10; H-IP-10; IFI10; Inp10; interferon activated gene 10; interferon-gamma induced protein CRG-2; interferon-gamma-induced protein CRG-2; interferon-gamma-inducible protein 10; interferon-gamma-inducible protein-10; interferon-inducible cytokine IP-10; interferon-inducible protein 10; IP10; IP-10; M-IP-10; Mob1; mob-1; protein 10 from interferon (gamma)-induced cell line; protein Mob-1; Scyb10; similar to Small inducible cytokine B10 precursor (CXCL10) (10 kDa interferon-gamma induced protein) (Gamma-IP10) (IP-10); small inducible cytokine B subfamily (Cys-X-Cys) member 10; small inducible cytokine B subfamily (Cys-X-Cys), member 10; small inducible cytokine subfamily B (Cys-X-Cys), member 10; small-inducible cytokine B10; C7; IFI10; INP10; IP-10; SCYB10; crg-2; gIP-10; mob-1
• Gene ID (Entrez): 3627
• Gene Symbol: CXCL10
• Interassay CV: 10.4%
• Intraassay CV: 6.7%
• Kit Contents: Pre-coated 96 well Microplate, Biotinylated Detection Antibody, SAV-HRP, Sample Diluent, lyophilized, Standard (8-well strips x 4), Wash Buffer Concentrate, Substrate Solution, Sample Diluent, Stop Solution, Lysis Buffer 10x, Adhesive Plate Covers, Biotinylated Detection Antibody, SAV-HRP, Sample Diluent, lyophilized, Standard (8-well strips x 4), Wash Buffer Concentrate, Substrate Solution, Sample Diluent, Stop Solution, Lysis Buffer 10x, Adhesive Plate Covers
• Label or Dye: Biotin
• Protein Aliases: 10 kDa interferon gamma-induced protein, Gamma-IP10, IP-10, Small-inducible cytokine B10
• Quantity: 128 Tests
• Regulatory Status: RUO
• Sample Volume: Amniotic Fluid 10 μL, Plasma 10 μL, Serum 10 μL, Supernatant 10 μL, Synovial Fluid 10 μL, Urine 10 μL
• Storage Requirements: -20°C
• Synonym: Interferon-gamma-inducible protein 10, CXCL10
• Target: CXCL10 (IP-10)
• Test Time: 15 min
• Total Assay Time: 3 hr 10 min