Thermo Scientific™ IPTG Solution, ready-to-use

Catalog No.	Quantity
FERR1171	10 x 1.5 mL

Catalog No. FERR1171 Supplier Thermo Scientific™ Supplier No. R1171

Description

Induce the expression of genes under the control of lac operon in cloning and protein expression procedures.

IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. IPTG is used to induce expression of cloned genes under control of the lac operon . It is used in conjunction with X-Gal (#R0941) to determine the lac phenotype in blue/white colony screening.

It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. IPTG is used to induce expression of cloned genes under control of the lac operon. It is used in conjunction with X-Gal to determine the lac phenotype in blue/white colony screening.

Ready-to-use 100mM IPTG aqueous solution is non-toxic, stable, 0.22μm membrane-filtered, formulated for direct use in blue/white colony screening.

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

Specifications

• Chemical Name or Material: Isopropylthiogalactoside
• Concentration: 0.1 M
• For Use With (Application): Protein Expression
• Product Type: IPTG Solution
• Quantity: 10 x 1.5 mL
• Form: Liquid

Frequently Asked Questions (FAQs)

How do I resuspend IPTG and X-Gal?

IPTG can be reconstituted in water. Make a stock of 100 mM in water and store working aliquots at -20°C. X-gal can be reconstituted in DMSO, or in a 50:50 mix of DMSO and water. To do the latter, you must dissolve in DMSO first, and then add water to bring up to final volume. It is not necessary to filter sterilize these solutions.

The X-gal solution should be protected from light. To make plates, add 50 ug/ml X-gal and 1 mM (0.24 mg/mL) IPTG to LB/agar that has been cooled down to 50°C. To spread on top of plates, use 50 µl 2% stock of X-gal and 30 µl 100 mM stock of IPTG. 

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.