Thermo Scientific™ IPTG 

Induce the expression of genes under the control of lac operon in cloning and protein expression procedures.

Manufacturer:  Thermo Scientific™ R1171

Catalog No. FERR1171

Description

IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. IPTG is used to induce expression of cloned genes under control of the lac operon . It is used in conjunction with X-Gal (#R0941) to determine the lac phenotype in blue/white colony screening.

It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. IPTG is used to induce expression of cloned genes under control of the lac operon. It is used in conjunction with X-Gal to determine the lac phenotype in blue/white colony screening.

Ready-to-use 100mM IPTG aqueous solution is non-toxic, stable, 0.22µm membrane-filtered, formulated for direct use in blue/white colony screening.

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Specifications

• For Use With (Application): Blue/white colony screening to distinguish recombinant (white) from non-recombinant (blue) colonies (1); Induction of gene expression under control of the lac promoter.
• Product Type: IPTG Solution, ready-to-use
• Quantity: 10 x 1.5mL (Aqueous Solution: 100mM)