Edvotek™ Isolation of E. Coli Chromosomal DNA
Isolate chromosomal DNA from E. Coli.
Supplier: Edvotek™ 203
DescriptionIsolation of high molecular weight chromosomal DNA is the first step in molecular cloning since it is the source of genes in cells. This experiment provides DNA Extraction LyphoCells™ and reagents for isolating chromosomal DNA from E. coli. After spooling from solution, the DNA can be dissolved and analyzed by agarose gel electrophoresis as an optional lab extension activity.
|Isolating chromosomal DNA|
|20 DNA Isolations and 5 Gels|
|1 hour 45 minutes|
|Instructions, Chromosomal LyphoCells™, Various solutions and buffers, Agarose powder, FlashBlue™ stain|
|Water bath, pipet pumps or bulbs, lab glassware, distilled or deionized water, 95-100% isopropanol. For Optional Electrophoresis: Electrophoresis apparatus, power supply, automatic micropipet with tips, balance, microwave oven or hot plate, misc. labware, white light visualization system, and photodocumentation system.|
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