Isolithocholic acid (CAS No 1534-35-6) is a secondary bile acid derived from intestinal microbial metabolism of primary bile acids It is primarily researched in the field of gastroenterology and hepatology due to its involvement in liver metabolism and potential implications in liver diseases The compound interacts with nuclear receptors such as FXR (farnesoid X receptor) and TGR5 (G protein-coupled bile acid receptor 1) influencing pathways related to metabolism and inflammation Isolithocholic acid exhibits activity in the nanomolar to low micromolar range making it a potent compound for experimental studies It is typically utilized in various in vitro assays including cell-based models and in vivo animal studies to investigate its effects on liver function cholesterol regulation and potential therapeutic applications Experimental concentrations typically depend on the specific research design employed in these studies