i Taq /i DNA Polymerase is a thermostable DNA polymerase isolated from an i E coli /i strain that carries the i Taq /i DNA polymerase gene i Taq /i DNA Polymerase is the most common polymerase used in PCR In some cases such as RAPD PCR adding large volume of general i Taq /i DNA polymerase (5 U/ l) which has a high concentration of glycerol in its storage buffer will increase the glycerol concentration in the reaction mix interfering with PCR performance The use of concentrated i Taq /i DNA Polymerase (25 U/ l ) with a far slimmer dose of glycerol can prevent poor PCR efficiency br br b Note /b Concentrated i Taq /i DNA Polymerase (GenScript E00012) is supplied with 10X reaction buffer containing 15 mM magnesium chloride The a href https //www genscript com/enzyme/D0056-dNTP mixture 10 mM each html dNTP (10 mM) mix /a may be ordered separately