Invitrogen™ Human Kallikrein 10 ELISA Kit

Catalog No.	Quantity
EHKLK10X10	10 x 96 Tests

Catalog No. EHKLK10X10 Supplier Invitrogen™ Supplier No. EHKLK10X10

Description

ELISA

The Human Kallikrein 10 (Hu KLK10) ELISA quantitates Hu KLK10 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu KLK10. Principle of the method The Human KLK10 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its encoded protein is secreted and may play a role in suppression of tumorigenesis in breast and prostate cancers. Alternate splicing of this gene results in multiple transcript variants encoding the same protein.

Specifications

• Accession Number: O43240
• Assay Range: 2.46-600 ng/mL
• Assay Sensitivity: 2 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Cell Structure/Organelle Proteins
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 2300002A13Rik; breast normal epithelial cell associated serine protease; kallikrein 10; kallikrein 10 protein 1; kallikrein 10 protein 12; kallikrein 10 protein 13; kallikrein 10 protein 2; kallikrein 10 protein 3; kallikrein 10 protein 4; kallikrein 10 protein 5; kallikrein 10 protein 7; kallikrein 10 protein 8; kallikrein 10 protein 9; kallikrein related peptidase 10; kallikrein related-peptidase 10; kallikrein-10; kallikrein-related peptidase 10; Klk10; NES1; Normal epithelial cell-specific 1; protease serine-like 1; protease, serine-like, 1; PRSSL12300002A13Rik; breast normal epithelial cell associated serine protease; kallikrein 10; kallikrein 10 protein 1; kallikrein 10 protein 12; kallikrein 10 protein 13; kallikrein 10 protein 2; kallikrein 10 protein 3; kallikrein 10 protein 4; kallikrein 10 protein 5; kallikrein 10 protein 7; kallikrein 10 protein 8; kallikrein 10 protein 9; kallikrein related peptidase 10; kallikrein related-peptidase 10; kallikrein-10; kallikrein-related peptidase 10; Klk10; NES1; Normal epithelial cell-specific 1; protease serine-like 1; protease, serine-like, 1; PRSSL1; NES1; PRSSL1
• Gene ID (Entrez): 5655
• Gene Symbol: KLK10
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Protein Aliases: Normal epithelial cell-specific 1, Protease serine-like 1
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 20 μL, Serum 20 μL, Supernatant 100 μL
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: Kallikrein 10
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min