Invitrogen™ Human Kallikrein 10 ELISA Kit

Description

ELISA

The Human Kallikrein 10 (Hu KLK10) ELISA quantitates Hu KLK10 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu KLK10. Principle of the method The Human KLK10 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its encoded protein is secreted and may play a role in suppression of tumorigenesis in breast and prostate cancers. Alternate splicing of this gene results in multiple transcript variants encoding the same protein.

Specifications

• Accession Number: O43240
• Assay Range: 2.46 to 600 ng/mL
• Assay Sensitivity: 2 ng/mL
• Calibration Range: 2 ng/mL to 600 ng/mL
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Product Type: ELISA
• Protein Family: Cell Structure & Organelle Proteins
• Sample Type: Plasma, Serum, Supernatant
• Specificity: This ELISA pair antibody detects human KLK10. Other species not determined.
• Sufficient For: 96 Assays
• Target Species: Human
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 5655
• Gene Symbol: NES1,PRSSL1
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Research Discipline: Cell Cycle & Proliferation
• Sample Volume: Plasma 20 μL, Serum 20 μL, Supernatant 100 μL
• Storage Requirements: 2°C to 8°C
• Synonym: NES1, PRSSL1
• Target: Kallikrein 10
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins