ProSci KillerTRAIL, Soluble Recombinant Protein

Catalog No.	Quantity
16-835-020	50 μg

Catalog No. 16-835-020 Supplier ProSci Supplier No. 903350050

Description

Recombinant Protein

This recombinant proteins is for research use only.

TNF-related apoptosis-inducing ligand (TRAIL; Apo2L;CD253; TNFSF10) is a type II transmembrane protein of about 34kDa. Like most members of the tumor necrosis factor (TNF) superfamily of cytokines TRAIL can be cleaved at the cell surface by metalloproteases to form a soluble molecule. Active TRAIL forms trimers and specifically binds to five distinct receptors: TRAIL-R1 (DR4; Apo2;CD261; TNFRSF10A), TRAIL-R2 (DR5; KILLER; TRICK2A;TRICK2B; CD262; TNFRSF10B), TRAIL-R3 (DcR1;LIT; TRID; CD263; TNFRSF10C), TRAIL-R4 (DcR2;TRUNDD; CD264; TNFRSF10D), and osteoprotegerin (OPG; OCIF; TNFRSF11B). Trimerized TRAIL triggers apoptosis upon ligation of cell surface TRAIL-R1 and/or TRAIL-R2 by inducing the formation of the so-called multiprotein death-inducing signaling complex (DISC).

Specifications

• Accession Number: P50591
• Concentration: 0.5 mg/mL
• Gene ID (Entrez): 8743
• Quantity: 50 μg
• Source: E. coli
• Common Name: tumor necrosis factor (ligand) superfamily, member 10
• Gene Symbol: TNFSF10
• Biological Activity: Potent biologically active trimer. Induces apoptosis in a concentration range of 10-100 ng/mL Note: Does not require a cross-linking enhancer for its potent biological activity. ED50: 20 ng/mL (BJAB cells). For cell lines that require extensive cross-linking of the TRAIL-Rs for killing (e.g. Jurkat) use SuperKillerTRAILTM (Prod. No. AG-40 T-0002).
• Conjugate: Unconjugated
• Species: Human, Mouse
• Recombinant: Recombinant
• Protein Tag: His Tag
• Sequence: The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide.
• Content And Storage: Stable for at least 1 year after receipt when stored (at -80°C.)
• Purity or Quality Grade: >98% (SDS-PAGE). Endotoxin level is less than 0.01 EU/ μg purified protein (LAL test; Lonza).
• Synonym: TRAIL, Apo-2 L, TNFSF10, CD253