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Thermo Scientific™ Kinase Tracer 222, 50 μM
Description
The LanthaScreen™ Eu Kinase Binding Assay
• Is easily optimized
• Measures inhibitor binding
• Performs simple off-rate measurements
Easily Screen for Kinase Binding
The LanthaScreen™ Eu Kinase Binding Assay is a TR-FRET assay that lets you measure inhibitor binding to non-activated kinases, perform simple off-rate measurements, and makes simple the study of type III (allosteric) inhibitors.
The LanthaScreen™ Eu Kinase Binding Assay is easy to optimize and run. The assay uses a simple mix-and-read format with a single one hour incubation and no development steps. Binding of an Alexa Fluor™ conjugate or “tracer” to a tagged kinase is detected by addition of a europium-labeled anti-tag antibody, in this case anti-GST. Simultaneous binding of the tracer and antibody to the kinase results in a high degree of fluorescence resonance energy transfer (TR-FRET), while displacement of the tracer with a kinase inhibitor results in a loss of TR-FRET.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Specifications
Specifications
| Format | 384-well plate |
| For Use With (Application) | TR-FRET |
| Quantity | 25 μL |
| Product Type | Kinase Tracer 222 |
| Content And Storage | Store at -20°C |
| Detection Method | Fluorescence |
| For Use With (Equipment) | Microplate Reader |
| Readout | Kinetic |
| Shipping Condition | Dry Ice |
| Target | Kinases (Multiple) |
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Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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