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Thermo Scientific™ Kinase Tracer 222, 50 μM

Catalog No. PV6121
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PV6121 25 μL
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Description

Kinase Tracer 222 is an Alexa Fluor™ 647 dye-labeled, ATP-competitive kinase inhibitor scaffold for use in the LanthaScreen™ Eu Kinase Binding Assay.

The LanthaScreen™ Eu Kinase Binding Assay
• Is easily optimized
• Measures inhibitor binding
• Performs simple off-rate measurements

Easily Screen for Kinase Binding
The LanthaScreen™ Eu Kinase Binding Assay is a TR-FRET assay that lets you measure inhibitor binding to non-activated kinases, perform simple off-rate measurements, and makes simple the study of type III (allosteric) inhibitors.

The LanthaScreen™ Eu Kinase Binding Assay is easy to optimize and run. The assay uses a simple mix-and-read format with a single one hour incubation and no development steps. Binding of an Alexa Fluor™ conjugate or “tracer” to a tagged kinase is detected by addition of a europium-labeled anti-tag antibody, in this case anti-GST. Simultaneous binding of the tracer and antibody to the kinase results in a high degree of fluorescence resonance energy transfer (TR-FRET), while displacement of the tracer with a kinase inhibitor results in a loss of TR-FRET.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

TRUSTED_SUSTAINABILITY

Specifications

Format 384-well plate
For Use With (Application) TR-FRET
Quantity 25 μL
Product Type Kinase Tracer 222
Content And Storage Store at -20°C
Detection Method Fluorescence
For Use With (Equipment) Microplate Reader
Readout Kinetic
Shipping Condition Dry Ice
Target Kinases (Multiple)
Label or Dye Alexa Fluor™ 647
Color Red
Product Line LanthaScreen
Emission 665
Excitation 340
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Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

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