Invitrogen™ Human KIT [D816V], GST Tag, His Tag Recombinant Protein

Catalog No.	Quantity
PV6201	1 mg
PV6200	100 μg
PV6199	10 μg

Catalog No. PV6201 Supplier Invitrogen™ Supplier No. PV6201

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Human KIT, C-terminal fragment, amino acids T544-V976 (as in NCBI/Protein entry NP_000213.1) with a D816V mutation, N-terminal GST-HIS6 fusion protein with a 3C cleavage site, expressed in Sf9 insect cells. , Validated in LanthaScreen™ Kinase Binding Assay, Protocols available for simple use and optimized performance, Multiple pack sizes available for convenient use Related Product: LanthaScreen™ Eu Kinase Binding Assay Protein Form: Recombinant

c-Kit, also known as mast/stem cell growth factor receptor (SCFR) or CD117, is a trans membrane type III tyrosine kinase receptor encoded by the c-Kit proto-oncogene, located on chromosome 4 in humans. It is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells. Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.

Specifications

• Concentration: See Label
• For Use With (Application): Kinase Assay
• Format: Kit
• Formulation: 50mM HEPES with 5mM DTT, 20% glycerol, 15mM glutathione, 100mM NaCl and no preservative; pH 7.5
• Molecular Weight (g/mol): 77.4 kDa
• Name: Human KIT [D816V], GST Tag, His Tag
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 1 mg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: CD117, PBT, SCFR, c-Kit
• Common Name: KIT (D816V Mutant)
• Protein Length: aa 544-976
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag, His-tag
• Sequence: 544-976
• Content And Storage: Storage Buffer: 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol
  
  Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws.
• Kinase Group: Receptor Tyrosine Kinase
• Expression System: Insect cells
• Protein Form: Recombinant
• Form: Liquid