Invitrogen™ Human KIT [N822K], GST Tag Recombinant Protein

Catalog No.	Quantity
PV6310	10 μg
PV6311	100 μg
PV6312	1 mg

Catalog No. PV6310 Supplier Invitrogen™ Supplier No. PV6310

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Recombinant human KIT N822K (544-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. , Validated in LanthaScreen™ Kinase Binding Assay, Protocols available for simple use and optimized performance, Multiple pack sizes available for convenient use Related Product: LanthaScreen™ Eu Kinase Binding Assay Protein Form: Recombinant

KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT together with its ligand regulates growth and activation of a variety of hemopoietic and non-hemopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Recently, deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has served as a focal point in therapeutic strategies aimed at curbing neoplastic mast cell growth. c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells. Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.

Specifications

• Concentration: See Label
• For Use With (Application): Kinase Assay
• Format: Kit
• Formulation: 50mM tris HCl with 0.25mM DTT, 25% glycerol, 0.1mM EGTA, 0.1mM PMSF, 150mM NaCl, 0.1mM EDTA and no preservative; pH 7.5
• Molecular Weight (g/mol): 75.3 kDa
• Name: Human KIT [N822K], GST Tag
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 10 μg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: PBT, SCFR, CD117
• Common Name: KIT (N822K Mutant)
• Protein Length: aa 544-end
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag
• Sequence: 544-end
• Content And Storage: Storage Buffer: 50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.1 mM EGTA, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol
  
  Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws.
• Assay Entry: Biochemical binding/kinetic
• Kinase Group: Receptor Tyrosine Kinase
• Target Entry: KIT N822K
• Expression System: Insect cells
• Protein Form: Recombinant
• Form: Liquid