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New England Biolabs, Inc. KLD Enzyme Mix – 25 reactions
Supplier: New England Biolabs, Inc. M0554S
KLD Enzyme Mix is a unique blend of Kinase, Ligase and DpnI enzymes. This formulation allows efficient phosphorylation, intramolecular ligation/circularization and template removal in a single 5 minute reaction step at room temperature. This master mix is a component of the Q5 Site-Directed Mutagenesis Kits and it has been designed for use with fragments that have been PCR-amplified by Q5 Hot Start High-Fidelity DNA Polymerase.
- Ability to phosphorylate and ligate in a single step
- Degradation of template DNA by DpnI
- Fast reaction time (5 minutes)
- Combination of 3 enzymatic activities (kinase, ligase, DpnI) in a single enzyme mix
- Also available as a kit: with and without competent cells
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