Enzo Life Sciences Klenow (3'-5' exo-) (20µl)

Supplier:  Enzo Life Sciences ENZ44001U020

Klenow (3′-5′ exo-) is a mesophilic DNA polymerase deficient in both proofreading (3′-5′) and nick-translation (5′-3′) nuclease activities, and that displays a moderate strand displacement activity during DNA synthesis. The protein is expressed as a truncated product of the E.coli PolA gene and contains the D355A and E357A mutations. Klenow is a mesophilic DNA polymerase deficient in both proofreading (3′-5′) and nick-translation (5′-3′) nuclease activities, and that displays a moderate strand displacement activity during DNA synthesis. Alternative name: Klenow fragment. Concentration: Unit Concentration: 50 U/µl Protein Concentration: 5 mg/ml. Purity: ≥99% (SDS-PAGE). Formulation: Liquid. In 20mM TRIS-HCl, pH 7.5, containing 50% glycerol, 0.1mM EDTA and 1mM DTT. Source: Recombinant E. coli. carrying the Klenow gene. Specificity: SS Exonuclease: 500U <0.1% released DS Exonuclease: 500U <0.1% released Endonuclease: 500U <0.1% converted E. coli 16S rDNA Contamination 500U <10 copies.

Catalog No. 50-201-2560