Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. NC4007594

AURORA BIOLABS LLC Kras G12A Nucleotide Exchange Assay Kit, 100 rxn

Catalog No. NC4007594
Encompass_Preferred

The KRAS G12A Nucleotide Exchange Assay Kit is a TR-FRET–based assay designed to measure the nucleotide exchange activity of the KRAS G12A mutant protein. KRAS is a small GTPase involved in Ras signaling pathways that regulate cell growth, proliferation, and differentiation. Mutations such as G12A reduce GTPase activity and maintain KRAS in an active GTP-bound state, contributing to cancer progression. In this assay, Tag2-KRAS G12A is recognized by a terbium-labeled anti-Tag2 antibody (HTRF donor). Binding of fluorescently labeled GTP (HTRF acceptor) brings donor and acceptor into close proximity, enabling FRET upon excitation at 340 nm and emission at 665 nm. The fluorescence ratio (665/620 nm) quantitatively reflects GTP binding activity. Compounds that inhibit nucleotide exchange reduce the HTRF signal, making the kit suitable for screening KRAS G12A inhibitors.

Catalog No. NC4007594 Supplier AURORA BIOLABS LLC Supplier No. 5727412ANK100
May include imposed supplier surcharges.
Add to Cart
Add to Cart

missing translation for 'validMainframeMsg'

The KRAS G12A Nucleotide Exchange Assay Kit is a TR-FRET–based assay designed to measure the nucleotide exchange activity of the KRAS G12A mutant protein. KRAS is a small GTPase involved in Ras signaling pathways that regulate cell growth, proliferation, and differentiation. Mutations such as G12A reduce GTPase activity and maintain KRAS in an active GTP-bound state, contributing to cancer progression. In this assay, Tag2-KRAS G12A is recognized by a terbium-labeled anti-Tag2 antibody (HTRF donor). Binding of fluorescently labeled GTP (HTRF acceptor) brings donor and acceptor into close proximity, enabling FRET upon excitation at 340 nm and emission at 665 nm. The fluorescence ratio (665/620 nm) quantitatively reflects GTP binding activity. Compounds that inhibit nucleotide exchange reduce the HTRF signal, making the kit suitable for screening KRAS G12A inhibitors.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.