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AURORA BIOLABS LLC Kras G12C Binding Assay, 384 rxn
Supplier: AURORA BIOLABS LLC 57274122BK384
The Aurora Biolabs KRAS G12C c-RAF Binding Assay Kit is a TR-FRET based assay used to quantify binding between active KRAS G12C and the Ras Binding Domain (RBD) of c-RAF. The KRAS protein is preloaded with GTP and recognized by a terbium-labeled anti-Tag2 antibody (HTRF donor), while the c-RAF-RBD is labeled with a fluorescent acceptor. Upon binding, FRET occurs and emission at 665nm is measured. This assay provides a robust method for screening inhibitors that block KRAS effector interactions. Ideal for oncology research and drug discovery, each kit contains reagents for 384 reactions.
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