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KRISHGEN BIOSYSTEMS Enzymatic Assay for Quantification of Hyaluronidase activity in cell culture supernatant and pharmaceutical preparations. Assay Range: 28 U/ml – 896 U/ml. Pack size: 200 tests.
Supplier: KRISHGEN BIOSYSTEMS KBBA05200 TESTS
Pack Size: 1 x 96 wells. The KRISHZYME Hyaluronidase Assay kit uses a two-step turbidimetric reaction to measure hyaluronidase activity by the amount of hyaluronic acid that is hydrolyzed. A stop reagent halts the enzymatic reaction and forms turbidity with any residual hyaluronic acid in the well. The decrease in turbidity at 600 nm is directly proportional to hyaluronidase activity in the sample.Hyaluronidase is a family of enzymes that degrade hyaluronic acid by catalyzing the hydrolysis of hyaluronan, a constituent of the interstitial barrier. Hyaluronidase lowers the viscosity of hyaluronan, thereby increasing tissue permeability. It is, therefore, used in medicine in conjunction with other drugs to speed their dispersion and delivery. This assay accurately quantifies hyaluronidase activity in cell culture and pharmaceutical samples. Lot to lot consistency ensures accurate results for batch releases.
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