Recombinant Protein;Human LAG-3 / CD223 Protein, Mouse IgG2a Fc Tag;1MG;Human LAG-3, mouse IgG2a Fc tag (LA3-H52Aa) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Leu 450 (Accession # P18627-1).;This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 73.3 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Anti-Human LAG3 MAb, Human IgG1 at 0.5 μg/mL (100 μL/well) can bind Human LAG-3, mouse IgG2a Fc tag (Cat. No. LA3-H52Aa) with a linear range of 0.8-3 ng/mL (QC tested).;Flow Cytometry assay shows that recombinant human LAG3, Mouse IgG2a Fc Tag (Cat. No. LA3-H52Aa ) can bind to daudi cell surface. The concentration of LAG3 mFc used is 0.3μg/ml (Routinely tested).;FACS analysis shows that the binding Human LAG3, Mouse IgG2a Fc Tag to daudi cells was inhibited by increasing concentration of neutralizing anti-LAG3 antibody. The concentration of LA