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ACROBiosystems Recombinant Protein;Biotinylated Human LAG-3 / CD223 Protein, Mouse IgG2a Fc Tag, Avi Tag (Avitag™);HEK293;200UG;LA3,LAG3,CD223,LAG-3,FDC
Supplier: ACROBiosystems LA3H82F3200UG
Recombinant Protein;Biotinylated Human LAG-3 / CD223 Protein, Mouse IgG2a Fc Tag, Avi Tag (Avitag™);200UG;Biotinylated Human LAG-3, Mouse IgG2a Fc Tag, Avi Tag (LA3-H82F3) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Leu 450 (Accession # P18627-1).;This protein carries a mouse IgG2a Fc tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 75.4 kDa. The protein migrates as 100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Madarex LAG-3 Mab,Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human LAG-3, Mouse IgG2a Fc Tag, Avi Tag (Cat. No. LA3-H82F3) with a linear range of 0.4-13 ng/mL (QC tested).;Flow Cytometry assay shows that Biotinylated Human LAG-3, Mouse IgG2a Fc Tag, Avi Tag (Cat. No. LA3-H82F3) can bind to daudi cell surface. The concentration of LAG-3 used is 0.3 μg/ml (Routinely tested).
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