Invitrogen™ LanthaScreen™ TR-FRET BACE1 Assay Kit

Description

BACE1 (beta-secretase) is a key enzyme involved in the production of amyloid beta-peptides found in extracellular amyloid plaques of Alzheimer's disease (AD).

BACE1 (beta-secretase) is a key enzyme involved in the production of amyloid beta-peptides found in extracellular amyloid plaques of Alzheimer's disease (AD). In some cases, early-onset familial AD can be attributed to a 'Swedish' mutation in the amyloid precursor protein (APP), which dramatically enhances the cleavage of this protein by BACE1. This and other genetic and pathological evidence has led to therapeutic approaches that focus on the inhibition of BACE1 and other APP-cleaving enzymes, such as gamma-secretase.

Invitrogen's LanthaScreen™ TR-FRET BACE1 assay provides sensitive high-throughput screening for potential inhibitors of beta-secretase. The kit uses a terbium (Tb)-labeled anti-biotin antibody and a fluorescein-labeled BACE1-biotin substrate in a homogeneous TR-FRET assay format (Figure 1).

Contents and Storage:

The LanthaScreen™ TR-FRET BACE1 Assay Kit contains BACE1 protein, fluorescently labeled BACE1 substrate, Tb-labeled anti-biotin antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).

Specifications

• Content And Storage: Store in freezer (-5°C to -30°C).
• Assay: β-Secretase Assay
• Product Line: LanthaScreen™
• Substrate Properties: Peptide-Based Substrate
• Substrate Type: Beta Secretase Substrate
• For Use With (Application): TR-FRET
• Quantity: 800 x 20 μL Assays
• Shipping Condition: Dry Ice
• Target: Beta Secretase (BACE1)

For Research Use Only. Not for use in diagnostic procedures.