Thermo Scientific™ LanthaScreen™ TR-FRET CAR Coactivator Assay Kit, goat

Catalog No.	Quantity
PV4836	800 x 20 μL assays

Catalog No. PV4836 Supplier Thermo Scientific™ Supplier No. PV4836

Description

The LanthaScreen™ TR-FRET Constitutive Androstane Receptor (CAR) Coactivator Assay provides a sensitive and robust method for highthroughput screening (HTS) of potential CAR ligands as agonists of ligand-dependent coactivator recruitment or inverse agonists of ligand-dependent coactivator displacement.

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15141:

The LanthaScreen™ TR-FRET Constitutive Androstane Receptor (CAR) Coactivator Assay provides a sensitive and robust method for highthroughput screening (HTS) of potential CAR ligands as agonists of ligand-dependent coactivator recruitment or inverse agonists of ligand-dependent coactivator displacement. The kit uses a terbium-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a CAR ligand-binding domain (CAR-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous mix-and-read assay format.

To assay:

When running the LanthaScreen™ TR-FRET CAR Coactivator Assay, CAR-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and terbium anti-GST antibody. After an incubation period at room temperature, the 520 nm/ 495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the CAR-coactivator peptide interaction, this ligand's EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and either recruit or displace coactivator peptide (Figures 1 and 2).

Specifications

• Conjugate: Tb (Terbium)
• Detection Method: Fluorescence
• For Use With (Equipment): Microplate Reader
• Quantity: 800 x 20 μL assays
• Packaging: 384-well Plate
• Content And Storage: The LanthaScreen™ TR-FRET CAR Coactivator Assay Kit contains CAR-LBD (GST) protein, fluorescently labeled PGC1a coactivator peptide, terbium-labeled anti-GST antibody, and buffers.
  
  Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).
• Assay Entry: Biochemical coregulator interaction
• Readout: End Point
• Shipping Condition: Dry Ice
• Target Entry: CAR, NR1I3
• Product Line: LanthaScreen
• For Use With (Application): Co-Factor Interaction Assay, TR-FRET
• Ligand: CAR
• Product Type: TR-FRET CAR Coactivator Assay Kit
• No. of Assays: 800 x 20 μL Assays

For Research Use Only. Not for use in diagnostic procedures.