Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Immunohistochemistry Kits and Reagents
  5. PV4800

Thermo Scientific™ LanthaScreen™ TR-FRET ERR beta Coactivator Assay Kit, goat

Catalog No. PV4800
Encompass_Preferred
Change view
Click to view available options
Quantity:
800 x 20 μL assays
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
PV4800 800 x 20 μL assays
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. PV4800 Supplier Thermo Scientific™ Supplier No. PV4800
Only null left
Add to Cart
Add to Cart

Description

The LanthaScreen™ TR-FRET ERR (estrogen related receptor) beta Coactivator Assay Kit provides a sensitive and robust method for highthroughput screening of potential ERR beta ligands as inverse agonists of ligand-dependent coactivator displacement.

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15139:

The LanthaScreen™ TR-FRET ERR (estrogen related receptor) beta Coactivator Assay Kit provides a sensitive and robust method for highthroughput screening of potential ERR beta ligands as inverse agonists of ligand-dependent coactivator displacement. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant ERR beta ligand-binding domain (ERR beta-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous mix-and-read assay format.

To assay:

When running the LanthaScreen™ ERR beta TR-FRET Coactivator Assay, ERR beta-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound.

Based on the biology of the ERR beta-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and displace coactivator peptide (see Figure 1).

TRUSTED_SUSTAINABILITY

Specifications

Conjugate Tb (Terbium)
Detection Method Fluorescence
For Use With (Equipment) Microplate Reader
Quantity 800 x 20 μL assays
Packaging 384-well Plate
Content And Storage The LanthaScreen™ TR-FRET ERR beta Coactivator Assay Kit contains ERR beta-LBD (GST) protein, fluorescently labeled PGC1a coactivator peptide, Tb-labeled anti-GST antibody, and buffers.

Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).
Assay Entry Biochemical coregulator interaction
Readout End Point
Shipping Condition Dry Ice
Target Entry NR3B2, ERR2, ESRRB
Product Line LanthaScreen
For Use With (Application) Co-Factor Interaction Assay, TR-FRET
Ligand ERR beta
Product Type TR-FRET ERR Beta Coactivator Assay Kit
No. of Assays 800 x 20 μL Assays
Show More Show Less

For Research Use Only. Not for use in diagnostic procedures.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.