Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Immunohistochemistry Kits and Reagents
  5. A15140

Thermo Scientific™ LanthaScreen™ TR-FRET FXR Coactivator Assay Kit, rabbit

Catalog No. A15140
Encompass_Preferred
Change view
Click to view available options
Quantity:
800 x 20 μL assays
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
A15140 800 x 20 μL assays
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. A15140 Supplier Thermo Scientific™ Supplier No. A15140
Only null left
Add to Cart
Add to Cart

Description

The LanthaScreen™ TR-FRET FXR (farnesoid X receptor) Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential FXR ligands as agonists of coactivator recruitment.

This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4833:

The LanthaScreen™ TR-FRET FXR (farnesoid X receptor) Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential FXR ligands as agonists of coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein- labeled coactivator peptide, and an FXR ligand-binding domain (FXR-LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous, mix-and-read assay format.

To assay:

When running the LanthaScreen™ TR-FRET FXR coactivator assay in agonist mode, FXR-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and used to determine the EC50 from a dose response curve of the compound. Based on the biology of the FXR-coactivator peptide interaction, the ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).

TRUSTED_SUSTAINABILITY

Specifications

Color Green
Conjugate Tb (Terbium)
Detection Method Fluorescence
For Use With (Equipment) Microplate Reader
Quantity 800 x 20 μL assays
Packaging 384-well Plate
Content And Storage • Tb-anti-GST Antibody (Rabbit) (store at -20°C)
• FXR-LBD, GST (-80°C)
• Fluoroscein Coactivator Peptide (-20°C)
• TR-FRET Coregulatore Buffer G (4°C)
• 1M DTT (-20°C or -80°C)
Assay Category Biochemical Assay
Druggable Target Nuclear Receptors
Readout TR-FRET (Activity)
Product Line LanthaScreen
For Use With (Application) Nuclear Receptor Assay, TR-FRET
Ligand FXR
Product Type TR-FRET FXR Coactivator Assay Kit
No. of Assays 800 x 20 μL
Show More Show Less

For Research Use Only. Not for use in diagnostic procedures.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.