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Thermo Scientific™ LanthaScreen™ TR-FRET LXR alpha Coactivator Assay Kit, rabbit

Catalog No. A15128
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Description

The LanthaScreen™ TR-FRET LXR (liver X receptor) alpha Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential LXR alpha ligands as agonists of coactivator recruitment.

This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4655:

The LanthaScreen™ TR-FRET LXR (liver X receptor) alpha Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential LXR alpha ligands as agonists of coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and an LXR alpha ligand-binding domain (LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous mix-and-read assay format.

In the LanthaScreen™ TR-FRET LXR alpha Coactivator Assay, LXR alpha-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the LXR alpha-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).

TRUSTED_SUSTAINABILITY

Specifications

Color Green
Conjugate Tb (Terbium)
Detection Method Fluorescence
For Use With (Equipment) Microplate Reader
Quantity 800 x 20 μL assays
Packaging 384-well Plate
Content And Storage • Tb-anti-GST Antibody (Rabbit) (store at -20°C)
• LXR alpha-LBD, GST (-80°C)
• Fluoroscein Coactivator Peptide (-20°C)
• TR-FRET Coregulatore Buffer H (4°C)
• 1M DTT (-20°C or -80°C)
Assay Category Biochemical Assay
Druggable Target Nuclear Receptors
Readout TR-FRET (Activity)
Product Line LanthaScreen
For Use With (Application) Nuclear Receptor Assay, TR-FRET
Ligand LXR alpha
Product Type TR-FRET LXR Alpha Coactivator Assay Kit
No. of Assays 800 x 20 μL Assays
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For Research Use Only. Not for use in diagnostic procedures.

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