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Invitrogen™ LanthaScreen™ TR-FRET LXR beta Coactivator Assay Kit, rabbit Non-distribution product as customer accommodation.

LanthaScreen™ TR-FRET LXR beta Coactivator Assay Kit (Rabbit)

Manufacturer:  Invitrogen™ A15129

Catalog No. A15129


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Description

Description

This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4658:

The LanthaScreen™ TR-FRET LXR (liver X receptor) beta Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential LXR beta ligands as agonists of coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and an LXR beta ligand-binding domain (LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous mix-and-read assay format.

How it works:
In the LanthaScreen™ TR-FRET LXR beta Coactivator Assay, LXR beta-LBD is added to ligand test compounds followed by the addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the LXR beta-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).

Contents and Storage:
The LanthaScreen™ TR-FRET LXR beta Coactivator Assay Kit contains LXR beta-LBD (GST) protein, fluorescently labeled D22 coactivator peptide, Tb-labeled anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).
Specifications

Specifications

Green
Fluorescent
Microplate reader
Tb-anti-GST antibody (rabbit), LXR beta-LBD, GST, fluorescein coactivator peptide, TR-FRET coregulator buffer I, 1M DTT
LanthaScreen™ TR-FRET LXR Beta Coactivator Assay Kit
For research use only
–80/–20/4°C
LanthaScreen™ TR-FRET LXR Beta Coactivator Assay Kit, 800 x 20μL Assays, Green, 384-Well Plate Format, Tb (Terbium) Dye Type, Fluorescent Detection Method, TR-FRET Technique, For Pharma & Biopharma, Target & Lead Identification & Validation
Pharma and biopharma, drug discovery and development, target and lead identification and validation, nuclear receptor biology, biochemical nuclear receptor assays, nuclear receptor coregulator interaction
384-well plate
LanthaScreen™ TR-FRET LXR Beta Coactivator Assay Kit
800 x 20μL Assays
800 x 20μL Assays
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For Research Use Only. Not for use in diagnostic procedures.