Thermo Scientific™ LanthaScreen™ TR-FRET TR alpha Coactivator Assay Kit, goat

Catalog No.	Quantity
PV4687	800 x 20 μL assays

Catalog No. PV4687 Supplier Thermo Scientific™ Supplier No. PV4687

Description

The LanthaScreen™ TR-FRET TR (thyroid receptor) alpha Coactivator Assay Kit provides a sensitive and robust method for highthroughput screening of potential TR alpha ligands as agonists of ligand-dependent coactivator recruitment.

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15136:

The LanthaScreen™ TR-FRET TR (thyroid receptor) alpha Coactivator Assay Kit provides a sensitive and robust method for highthroughput screening of potential TR alpha ligands as agonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant TR alpha ligand-binding domain (TR alpha-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous, mix-and-read assay format.

To Assay:

When performing the LanthaScreen™ TR-FRET TR alpha coactivator assay, TR alpha-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and used to determine the EC₅₀ from a dose response curve of the compound. Based on the biology of the TR alpha-coactivator peptide interaction, the ligand EC₅₀ is a composite value representing the amount of ligand required to bind to receptor, induce a conformational change, and recruit coactivator peptide (Figure 1).

Contents and Storage:

The LanthaScreen™ TR-FRET TR alpha Coactivator Assay Kit contains TR alpha-LBD (GST) protein, fluorescently labeled SRC2-2 coactivator peptide, Tb-labeled anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).

Specifications

• Conjugate: Tb (Terbium)
• Detection Method: Fluorescence
• For Use With (Equipment): Microplate Reader
• Quantity: 800 x 20 μL assays
• Packaging: 384-well Plate
• Content And Storage: Store in ultra-cold freezer (-68°C to -85°C).
• Assay Entry: Biochemical coregulator interaction
• Readout: End Point
• Shipping Condition: Dry Ice
• Target Entry: THRA, TR alpha, NR1A1
• Product Line: LanthaScreen
• For Use With (Application): Co-Factor Interaction Assay, TR-FRET
• Ligand: TR alpha
• Product Type: TR-FRET TR Alpha Coactivator Assay Kit
• No. of Assays: 800 x 20 μL

For Research Use Only. Not for use in diagnostic procedures.